RT Journal Article ID 64d1c695674f273d A1 Harada, Toshie A1 Kawaminami, Hiromi A1 Miura, Noriko N. A1 Adachi, Yoshiyuki A1 Nakajima, Mitsuhiro A1 Yadomae, Toshiro A1 Ohno, Naohito T1 Comparison of the Immunomodulating Activities of 1,3-β-glucan Fractions from the Culinary-Medicinal Mushroom Sparassis crispa Wulf.:Fr. (Aphyllophoromycetideae) JF International Journal of Medicinal Mushrooms JO IJM YR 2006 FD 2006-12-12 VO 8 IS 3 SP 231 OP 244 AB Sparassis crispa is a culinary-medicinal mushroom recently cultivated in Japan, China, Germany, and the U.S. In a previous study, we purified a 6-branched 1,3-β-D-glucan preparation, designated as SCG, and investigated its biological activities. In the present study, we prepared polysac-charide fractions extracted from S. crispa by hot water (SCHWE), cold NaOH (SCG), and hot NaOH (SCHA), and investigated their properties and immunomodulating activities. These polysaccharide fractions showed strong limulus factor G activity due to the presence of β-1,3-D-glucans. They also showed a strong antitumor activity against the solid form of Sarcoma 180 in ICR mice, and hemato-poietic response in cyclophosphamide-induced leukopenic mice. They induced the splenocytes from DBA/2 mice to produce GM-CSF, TNF-α, and IFN-γ in vitro. By treatment with sodium hydroxide of SCHWE, the hematopietic response was enhanced but cytokine induction was decreased. Taken together, these results strongly suggested that the preparation method was related to the conformation of the β-glucan fraction, and the immunomodulating activities in vivo and in vitro were significantly modulated by the preparation methods. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,67fa6af2515135b9,64d1c695674f273d.html