RT Journal Article
ID 61a779f542483460
A1 Angelini, Paola
A1 Tirillini, Bruno
A1 Bistocchi, Giancarlo
A1 Arcangeli, Andrea
A1 Rubini, Andrea
A1 Pellegrino, Roberto Maria
A1 Fabiani, Roberto
A1 Cruciani, Gabriele
A1 Venanzoni, Roberto
A1 Rosignoli, Patrizia
T1 Overview of the Biological Activities of a Methanol Extract from Wild Red Belt Conk, Fomitopsis pinicola (Agaricomycetes), Fruiting Bodies from Central Italy
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-11-02
VO 20
IS 11
SP 1047
OP 1063
K1 Fomitopsis pinicola
K1 antifungal
K1 antiradical
K1 antitumor
K1 LC/QTOF-MS
K1 medicinal mushrooms
K1 molecular typing
K1 Polyporales
AB Fomitopsis pinicola (Sw.) P. Karst. (Fomitopsidaceae) is a medicinal mushroom with a variety of healthy properties. In this study we tested the radical scavenging activity and antimicrobial and anticancer potential of methanol extracts of F. pinicola from central Italy. Molecular identification confirmed that the samples were F. pinicola; a Basic Local Alignment Search Tool search showed a close match (99% sequence identity) with European isolates of this species. The free radical scavenging capacities, measured by DPPH assay, showed that the extract activity was 3.5% that of Trolox. The MTT test, evaluated after 72 hours of treatment with increasing doses of extract (5-500 μg · mL-1), considerably inhibited proliferation in a dose-dependent manner in 2 human tumor cell lines. This reduction was coupled with a relevant induction of apoptosis in the human leukemia THP-1 cell line after 24 hours of treatment, but a relevant toxic effect occurred in the human colon adenocarcinoma HT29 cell line. The genotoxic potential of the methanol extracts was studied by single-cell gel electrophoresis of normal human leukocytes exposed to 20 μg extract at 37°C for 30 minutes; no DNA damage was observed. The F. pinicola methanol extract was found to have varying degrees of antifungal effects against the pathogenic fungi tested (minimum inhibitory concentration from 23.63 to 66.81 μg · mL-1). The results show that the tested F. pinicola extract has strong antimicrobial and chemo-preventive activities, but is a poor antioxidant.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,6e2de2a705b6ffd3,61a779f542483460.html