RT Journal Article ID 50a23ef213a04660 A1 Bae, In Young A1 Shin, Ji-Yoon A1 Lee, Hyeon Gyu T1 Preparation of Black Hoof Medicinal Mushroom Phellinus linteus (Berk. et M.A. Curt.) Teng (Aphyllophoromycetideae) (β-Glucan Sulfate and In Vitro Tumor Cell Growth Inhibitory Activity JF International Journal of Medicinal Mushrooms JO IJM YR 2011 FD 2011-05-11 VO 13 IS 2 SP 115 OP 120 K1 medicinal mushrooms K1 polysaccharides K1 Phellinus linteus K1 β-glucan K1 sulfation K1 nitric oxide K1 cytotoxic activity AB Polysaccharide β-glucans were extracted from the medicinal mushroom Phellinus linteus (Hymenochaetaceae, Aphyllophoromycetideae) and subjected to sulfation. Chemical modification of the β-glucan was confirmed by structural analysis, and its biological properties were compared with those of native β-glucan. The results of Fourier transform infrared spectroscopy and elemental analysis indicated that successive preparation of the sulfated derivative yielded a degree of substitution of 0.47. Nitric oxide production measured by the bronchoalveolar lavage (BAL) experiments increased 1.5-fold after sulfation. In addition, the introduction of sulfate groups into the β-glucan chains improved in vitro growth inhibitory activity against SNU-C2A cells. Therefore, sulfated β-glucan extracted from Ph. Linteus may be beneficial for immune support due to its incorporation of functional groups into its polymer structure. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,31e1791e300b7e1e,50a23ef213a04660.html