%0 Journal Article %A Bae, In Young %A Shin, Ji-Yoon %A Lee, Hyeon Gyu %D 2011 %I Begell House %K medicinal mushrooms, polysaccharides, Phellinus linteus, β-glucan, sulfation, nitric oxide, cytotoxic activity %N 2 %P 115-120 %R 10.1615/IntJMedMushr.v13.i2.30 %T Preparation of Black Hoof Medicinal Mushroom Phellinus linteus (Berk. et M.A. Curt.) Teng (Aphyllophoromycetideae) (β-Glucan Sulfate and In Vitro Tumor Cell Growth Inhibitory Activity %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,31e1791e300b7e1e,50a23ef213a04660.html %V 13 %X Polysaccharide β-glucans were extracted from the medicinal mushroom Phellinus linteus (Hymenochaetaceae, Aphyllophoromycetideae) and subjected to sulfation. Chemical modification of the β-glucan was confirmed by structural analysis, and its biological properties were compared with those of native β-glucan. The results of Fourier transform infrared spectroscopy and elemental analysis indicated that successive preparation of the sulfated derivative yielded a degree of substitution of 0.47. Nitric oxide production measured by the bronchoalveolar lavage (BAL) experiments increased 1.5-fold after sulfation. In addition, the introduction of sulfate groups into the β-glucan chains improved in vitro growth inhibitory activity against SNU-C2A cells. Therefore, sulfated β-glucan extracted from Ph. Linteus may be beneficial for immune support due to its incorporation of functional groups into its polymer structure. %8 2011-05-11