RT Journal Article ID 2418c7cd62f20bae A1 Berovic, Marin A1 Habijanic, Jozica A1 Boh, Bojana A1 Wraber, Branka A1 Petravic-Tominac, Vlatka T1 Production of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt. :Fr.) P. Karst. (Higher Basidiomycetes), Biomass and Polysaccharides by Solid State Cultivation JF International Journal of Medicinal Mushrooms JO IJM YR 2012 FD 2012-11-28 VO 14 IS 5 SP 513 OP 520 K1 medicinal mushrooms K1 Ganoderma lucidum K1 solid state cultivation K1 horizontal stirred tank reactor K1 fungal biomass K1 critical moisture content K1 polysaccharide production AB Solid state cultivation of Ganoderma lucidum biomass, strain BFWS Gal 4, originally isolated from the Slovenian forest, was studied in a horizontal stirred tank reactor. Periodic mixing of N = 80 rpm, 2 min/day was used. Production of fungal polysaccharides and fungal biomass on solid substrate based on beech sawdust, olive oil, and mineral salts was studied. Optimal moisture of the solid matrix was in the range of 80% to 74%. When the moisture content dropped below 57%, the growth of the mycelium and polysaccharide production stopped, but it revived when wet air was applied in further processing. Final concentration of biomass was 0.68 mg/g of solid substrate, while proportions of extracellular and intracellular polysaccharides were 4.5 mg/g and 1.05 mg/g, respectively. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,03ea8c440cfbb276,2418c7cd62f20bae.html