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Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal on Algae
SJR: 0.219 SNIP: 0.261 CiteScore™: 0.24

ISSN Печать: 1521-9429
ISSN Онлайн: 1940-4328

Выпуски:
Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal on Algae

DOI: 10.1615/InterJAlgae.v17.i4.50
pages 371-384

Features of Cell Metabolism of Chlamydomonas reinhardtii CC-124 Wild Strain [137c] Under Mixotrophic and Phototrophic Cultivation

O.V. Sytar
Department of Plant Physiology and Ecology, Institute of Biology, Taras Shevchenko National University of Kiev, 64, Vladimirskaia St., Kiev 01004, Ukraine
O. P. Olkhovich
Kiev National University Kiev, Ukraine
O.V. Karaushu
Department of Plant Physiology and Ecology, Institute of Biology, Taras Shevchenko National University of Kiev, 64, Vladimirskaia St., Kiev 01004, Ukraine
R. Storandt
Department of Biotechnology, IGV Institut fur Getreideverarbeitung GmbH, Arthur-Scheunert- Allee, 40/41, D-14558 Nuthetal OT Bergholz-Rehbrucke, Germany
P. Waldeck
Department of Biotechnology, IGV Institut fur Getreideverarbeitung GmbH, Arthur-Scheunert- Allee, 40/41, D-14558 Nuthetal OT Bergholz-Rehbrucke, Germany
Nataliya Yu. Taran
Research and Training Center "Institute of Biology and Medicine" of Kiev National Taras Shevchenko University, Kiev

Краткое описание

The features of cell metabolism were studied in Chlamydomonas reinhardtii CC-124 wild strain [137c]. It is a mutant for genes nit1 and nit2, characterized by insensitivity to the nitrate medium under phototrophic and mixotrophic cultivation. It was found that, in terms of chlorophyll and sulpholipid content in the cells of Ch. reinhardtii, phototrophic conditions of cultivation were the best. Total content of amino acids in Ch. reinhardtii had increased 1.5 times during the transition from photo- to mixotrophic nutrition. Among the studied amino acids, five are essential: valine, tryptophan, phenylalanine, methionine, and leucine. Under phototrophic conditions, quantitative content of essential amino acids increased nearly twice. The ratio of the amount of non-essential to essential amino acids during phototrophic nutrition equaled 3.27, while mixotrophic − 2.56. This indicates an increase of essential amino acids content and a better-balanced composition of amino acids under mixotrophic conditions of cultivation. The possibility of regulation of specific metabolites of Ch. reinhardtii CC-124 wild strain [137c] production by means of certain cultivation conditions is discussed.