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International Journal of Medicinal Mushrooms
Главный редактор: Solomon P. Wasser (open in a new tab)

Выходит 12 номеров в год

ISSN Печать: 1521-9437

ISSN Онлайн: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

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Expression of Hygromycin B Resistance in Oyster Culinary-Medicinal Mushroom, Pleurotus ostreatus (Jacq.:Fr.)P. Kumm. (Higher Basidiomycetes) Using Three Gene Expression Systems

Том 14, Выпуск 1, 2012, pp. 21-26
DOI: 10.1615/IntJMedMushr.v14.i1.20
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Краткое описание

Three hygromycin B phosphotransferase (hph) gene expression systems for culinary-medicinal Oyster mushroom, Pleurotus ostreatus, plasmid pSHC, pAN7-1, and pBHt1 were evaluated through PEG/CaCl2-mediated protoplast transformation. Plasmid pSHC is a newly constructed hph gene expression system, composed of Escherichia coli hph gene, the P. ostreatus sdi promoter, and the CaMV35S terminator. The vector pAN7-1 was commonly used for integrative transformation in filamentous fungi. Plasmid pBHtl is a T-DNA binary vector, usually introduced into fungi by Agrobacterium-mediated transformation. The results showed that plasmids pSHC, pAN7-1, and pBHt1 were all integrated into the host chromosomes and expressed hygromycin B resistance in P. ostreatus. pAN7-1 had the highest transformation efficiency and hph gene expression level, pSHC the second, and pBHt1 the lowest. Growth rates of the transformants on plates containing hygromycin B were in correspondence with their hph gene expression levels. To our knowledge, this is the first report on integrated transformation of plasmid pAN7-1 and pBHt1 in P. ostreatus.

ЦИТИРОВАНО В
  1. Chai Ran, Zhang Guang, Sun Qiang, Zhang Mingyue, Zhao Shuaiju, Qiu Liyou, Liposome-mediated mycelial transformation of filamentous fungi, Fungal Biology, 117, 9, 2013. Crossref

  2. Chai Ran, Qiu Cuiwei, Liu Dongren, Qi Yuancheng, Gao Yuqian, Shen Jinwen, Qiu Liyou, Stajich Jason E., β-Glucan Synthase Gene Overexpression and β-Glucans Overproduction in Pleurotus ostreatus Using Promoter Swapping, PLoS ONE, 8, 4, 2013. Crossref

  3. Liu Tianxiang, Li Huiru, Ding Yatong, Qi Yuancheng, Gao Yuqian, Song Andong, Shen Jinwen, Qiu Liyou, Genome-wide gene expression patterns in dikaryon of the basidiomycete fungus Pleurotus ostreatus, Brazilian Journal of Microbiology, 48, 2, 2017. Crossref

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