Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018027011
pages 717-726

Structural Characterization of Polysaccharides of a Productive Strain of the Culinary-Medicinal King Oyster Mushroom, Pleurotus eryngii (Agaricomycetes), from Italy

Francesca Cateni
University of Treiste, Faculty of Pharmacy Department of Chemical and Pharmaceutical Sciences P.zle Europa, 1, 34127 Treiste, Italy
Marina Zacchigna
Department of Chemical and Pharmaceutical Sciences, University of Trieste, Trieste, Italy
Celeste Caruso Bavisotto
Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy
Giuseppe Procida
Department of Chemical and Pharmaceutical Sciences, University of Trieste, Trieste, Italy
Giuseppe Bonaventura
Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy
Paola Saporita
Department of Agricultural, Food and Forest Sciences, University of Palermo, Palermo, Italy
Roberta Calvo
Department of Agricultural, Food and Forest Sciences, University of Palermo, Palermo, Italy
Giuseppe Venturella
Department of Agricultural, Food and Forest Sciences, University of Palermo, Palermo, Italy
Maria Letizia Gargano
Department of Agricultural and Forest Sciences University of Palermo Viale delle Scienze, Bld. 5, 90128 Palermo, Italy

Краткое описание

A preliminary biological investigation of the dry basidiomata of strain C-142-c of Pleurotus eryngii has shown significant antioxidant activity. Two different polysaccharides (PEPS-A1 and PEPS-A2) were isolated from the cultivated edible mushroom, P. eryngii C-142-c strain. Based on acid hydrolysis, methylation analysis, and nuclear magnetic resonance experiments (1H, 13C, distortionless enhancement by polarization transfer, double quantum filtered correlation spectroscopy, total correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single-quantum correlation spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structures of the repeating unit of PEPS-A1 and PEPS-A2 were established as follows: (l)PEPS-Al (α-glucan): [→6)-α-D-Glcp-(1→6)-α-D-Glcp-(l→]n; and (2) PEPS-A2 (β-glucan): [→6)-β-D-Glcp-(1→6)-β-D-Glcp-(l→]n. The antioxidant activity of PEPS-A1 and PEPS-A2 was evaluated as hydroxyl radical scavenging activity. PEPS-A1 and PEPS-A2 showed SC50 values of 400 μg/mL and 122 μg/mL, respectively, suggesting their possible use as a dietary supplement in functional foods. The polysaccharides were tested for their activity on cell viability using a colorectal adenocarcinoma cell line (HT-29). Both polysaccharides affected cell viability after 48 and 72 hours of treatment, inducing the death of 50% of HT-29 cells between 0.25 and 1 μg/mL and between 0.5 and 1 μg/mL, respectively, for PEPS-A1 and PEPS-A2. These results are promising for future applications of these mushroom-derived polysaccharides as antioxidants and antitumor agents.


Articles with similar content:

Characterization and Antiproliferative Effect of Novel Acid Polysaccharides from the Spent Substrate of Shiitake Culinary-Medicinal Mushroom Lentinus edodes (Agaricomycetes) Cultivation
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 5
Peixin He, Wei Liu, Yong Zhang, Chunping Xu, Wei Huang
Cytotoxic Activities of Funalia trogii (Berk.) Bond. Et. Singer ATCC 200800 Bioactive Extract on HeLa Cells and Fibroblast Cells
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Mehmet A. Mazmanci, Ali Unyayar, Murat Demirbilek, Ozlem Cekic, Melisa Turkoglu, Hatice Atacag, Serpil Unyayar, Emrah A. Erkurt, Ayla Celik
Did the Iceman Know Better? Screening of the Medicinal Properties of the Birch Polypore Medicinal Mushroom, Piptoporus betulinus (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 12
Maja Kozarski, Anita Klaus, Jovana Vunduk, Leonardus Johannes Lambertus Donatus Van Griensven, Predrag Petrovic, Miomir Niksic, Zeljko Zizak
Comparative Antitumor Activity of Jelly Ear Culinary-Medicinal Mushroom, Auricularia auricula-judae (Bull.) J. Schrot. (Higher Basidiomycetes) Extracts Against Tumor Cells In Vitro
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 4
Md.Ahsanur Reza, Seung-Chun Park, Woo-Sik Jo
Comparative Study on the Effect of Aqueous and Ethanolic Mycelial Extracts from Polycephalomyces nipponicus (Ascomycetes) against Human Breast Cancer MCF-7 Cells
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 7
Benjaporn Buranrat, Aphidech Sangdee, Kusavadee Sangdee