Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v16.i2.30
pages 125-135

Process Optimization for Extraction of Carotenoids from Medicinal Caterpillar Fungus, Cordyceps militaris (Ascomycetes)

Tao Yang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China; University of Chinese Academy of Sciences, Beijing, 100049, China
Junde Sun
College of Land and Environment, Shenyang Agricultural University, Shenyang, 110866, China
Tiantian Lian
College of Land and Environment, Shenyang Agricultural University, Shenyang, 110866, China; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
Wenzhao Wang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
Caihong Dong
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

Краткое описание

Natural carotenoids have attracted great attention for their important beneficial effects on human health and food coloring function. Cordyceps militaris, a well-known edible and medicinal fungus, is a potential source of natural carotenoids. The present study aimed to optimize the process parameters for carotenoid extraction from this mushroom. The effects of different methods of breaking the fungal cell wall and organic solvents were studied by the one-factor-at-a-time method. Subsequently, the process parameters including the duration of the extraction time, the number of extractions, and the solvent to solid ratio were optimized by using the Box-Behnken design. The optimal extraction conditions included using an acid-heating method to break the cell wall and later extracting three times, each for a 1 h duration, with a 4:1 mixture of acetone: petroleum ether and a solvent: solid ratio of 24:1. The carotenoid content varied from 2122.50 to 3847.50 µg/g dry weights in different commercially obtained fruit bodies of C. militaris. The results demonstrated that the C. militaris contained more carotenoid content in its fruit bodies than other known mushrooms. Stability monitoring by HPLC demonstrated that the carotenoids could be stored at 4°C for 40 d. It is suggested that the carotenoid content should be considered as the quality standard of commercial products of this valued mushroom. These findings will facilitate the exploration of carotenoids from C. militaris.


Articles with similar content:

Nutritional Requirements for Mycelial Growth of Milk-White Toothed Mushroom, Irpex lacteus (Agaricomycetes), in Submerged Culture
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 9
Xiaoming Dong, Xinhua Song, Caihong Dong
The Growing Effect of Vineyard and Winery Wastes on the Production of Mycelia and Fruit Bodies of Edible and Medicinal Fungi
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
George Dicu, Alexandru Teodorescu, Marian Petre
Preparation of Culinary-Medicinal Maitake Mushroom, Grifola frondosa (Dicks.: Fr.) S.F. Gray (Aphyllophoromycetideae)-fermented Wheat and its Antioxidant Properties
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 1
Shin-Yi Lin, Pei-Ying Lian, Jeng-Leun Mau, Shu-Yao Tsai, Chih-Hung Liang, Shih-Jeng Huang
An Axenic Culture System for Fruiting Body Formation by an Edible Bolete Phylogenetically related to Culinary-Medicinal Penny Bun Mushroom, Boletus edulis Bull.:Fr. Strains from China
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 4
Qi Tan, Ming-Jie Chen, Mei Yan Zhang, Xiaodong Shang, Shao Chun Fu
Determination of Lovastatin, β-glucan, Total Polyphenols, and Antioxidant Activity in Raw and Processed Oyster Culinary-Medicinal Mushroom, Pleurotus ostreatus (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 2
Yu Shan Lam , Edward J. Okello