Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
International Journal of Medicinal Mushrooms
Импакт фактор: 1.423 5-летний Импакт фактор: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344

Выпуски:
Том 22, 2020 Том 21, 2019 Том 20, 2018 Том 19, 2017 Том 18, 2016 Том 17, 2015 Том 16, 2014 Том 15, 2013 Том 14, 2012 Том 13, 2011 Том 12, 2010 Том 11, 2009 Том 10, 2008 Том 9, 2007 Том 8, 2006 Том 7, 2005 Том 6, 2004 Том 5, 2003 Том 4, 2002 Том 3, 2001 Том 2, 2000 Том 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019031922
pages 995-1006

Isolation, Anti-Inflammatory Activity and Physico-chemical Properties of Bioactive Polysaccharides from Fruiting Bodies of Cultivated Cordyceps cicadae (Ascomycetes)

Chun-Hsien Yang
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan
Chun-Han Su
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan
Shou-Chou Liu
Kang Jian Biotech Co., Ltd., Nantou, Taiwan
Lean-Teik Ng
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan

Краткое описание

Cordyceps cicadae is a medicinal fungus popularly used in traditional Chinese medicine for treating cancer, asthma, and kidney diseases. In this study, crude polysaccharides (CP) and water-soluble nondigestible polysaccharides (NDPs) were prepared from the fruiting bodies of cultivated C. cicadae, and their physicochemical properties and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were examined. The results showed yields of CP and NDP of 3.42% and 1.17%, respectively. CP and NDP showed a similar monosaccharide composition, of which the predominant monosaccharide was mannose, followed by galactose and glucose. Differences in molecular weight distribution between CP and NDP were apparent; CP possessed two major (3.1 kDa and 21.5 kDa) and one minor (678.2 kDa) macromolecular populations, whereas NDP contained only one macromolecular population (24.4 kDa). Furthermore, CP but not NDP had a triple helix conformation. The bioassay results showed that, although both CP and NDP possess anti-inflammatory activity, NDP had stronger inhibitory effects on nitric oxide, IL-1β, and TNF-α production in LPS-stimulated RAW264.7 macrophages. From this study, we conclude that the differences in conformation and molecular weight distribution between CP and NDP may contribute to their differences in anti-inflammatory activity.

ЛИТЕРАТУРА

  1. Weng SC, Chou CJ, Lin LC, Tsai WJ, Kuo YC. Immunomodulatory functions of extracts from the Chinese medicinal fungus Cordyceps cicadae. J Ethnopharmacol. 2002;83:79-85.

  2. Wang Q, Liu Z. Advances in studies on medicinal fungi Cordyceps cicadae. Chin Tradit Herbal Drugs. 2004;35:469-71.

  3. Zhu R, Chen YP, Deng YY, Zheng R, Zhong YF, Wang L, Du LP. Cordyceps cicadae extracts ameliorate renal malfunction in a remnant kidney model. J Zhejiang Univ Sci B. 2011;12:1024-33.

  4. Wang Q, Wang CL, He FG, Zhang HY. Research progress on native drug Isaria cicadae Miq. J Chin Oncol. 20i3;i9:227-30.

  5. Jin Z, Chen Y. Clinical observation on Cordyceps cicadae Shing Tang in preventing the progression of chronic renal failure. Chin Arch Tradit Chin Med. 2006;24:1457-59.

  6. Kim HS, Kim JY, Ryu HS, Shin BR, Kang JS, Kim HM, Kim YO, Hong JT, Kim Y, Han SB. Phenotypic and functional maturation of dendritic cells induced by polysaccharide isolated from Paecilomyces cicadae. J Med Food. 2011;14:847-56.

  7. Cai JF, Lu HY, Lu BZ. Research of anti-tumor effects of different purified components of Cordyceps cicadae in vitro. Chin Arch Tradit Chin Med. 2010;28:760-64.

  8. Sun Y, Wink M, Wang P, Lu H, Zhao H, Liu H, Wang S, Sun Y, Liang Z. Biological characteristics, bioactive components and antineoplastic properties of sporoderm-broken spores from wild Cordyceps cicadae. Phytomedicine. 2017;36:217-28.

  9. Wang H, Zhang J, Sit WH, Lee CY, Wan MF. Cordyceps cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells: a proteomic study. Chin Med. 2014;9:15. doi: 10.1186/1749-8546-9-15.

  10. Wang D, Wang J, Wang D, Yu X, Olatunji OJ, Ouyang Z, Wei Y. Neuroprotective effects of butanol fraction of Cordyceps cicadae on glutamate-induced damage in PC12 cells involving oxidative toxicity. Chem Biodivers. 2018;15:e1700385.

  11. Sharma SK, Gautam N. Chemical composition and antioxidant and antibacterial activities of cultured mycelia of four Clavi-cipitaceous mushrooms (Ascomycetes) from the Indian Himalayas. Int J Med Mushrooms. 2017;19:45-54.

  12. Hsu JH, Jhou BY, Yeh SH, Chen Yl, Chen CC. Healthcare functions of Cordyceps cicadae. J Nutr Food Sci. 2015;5:432. doi: 10.4172/2155-9600.1000432.

  13. Mizuno T. Medicinal effects and utilization of Cordyceps (Fr.) Link (Ascomycetes) and Isaria Fr. (mitosporic fungi) Chinese caterpillar fungi, "Tochukaso" (Review). Int J Med Mushrooms. 1999;3:251-61.

  14. Chen YL, Yeh SH, Lin TW, Chen CC, Chen CS, Kuo CF. A 90-day Subchronic toxicity study of submerged mycelial culture of Cordyceps cicadae (Ascomycetes) in rats. Int J Med Mushrooms. 2015;8:771-81.

  15. Giavasis I. Bioactive fungal polysaccharides as potential functional ingredients in food and nutraceuticals. Curr Opin Biotechnol. 2014;26:162-73.

  16. Wasser SP. Medicinal mushrooms in human clinical studies. Part I. anticancer, oncoimmunological, and immunomodulatory activities: a review. Int J Med Mushrooms. 2017;19:279-317.

  17. Su CH, Lai MN, Lin CC, Ng LT. Comparative characterization of physicochemical properties and bioactivities of polysaccharides from selected medicinal mushrooms. Appl Microbiol Biotechnol. 2016;100:4385-93.

  18. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28:350-56.

  19. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.

  20. Dai J, Wu Y, Chen SW, Zhu S, Yin HP, Wang M, Tang J. Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone. Carbo- hydr Polym. 2010;82:629-35.

  21. Ukai S, Matsuura S, Hara C, Kiho T, Hirose K. Structure of a new galactomannan from the ascocarps of Cordyceps cicadae Shing. Carbohydr Res. 1982;101:109-16.

  22. Kiho T, Miyamoto I, Nagai K, Ukai S, Hara C. A minor, protein-containing galactomannans from the insect-body portion of the fungal preparation Chan hua (Cordyceps cicadae). Carbohydr Res. 1988;181:207-15.

  23. Li SP, Li P, Dong TTX, Tsim KWK. Anti-oxidation activity of different types of natural Cordyceps sinensis and cultured Cordyceps mycelia. Phytomedicine. 2001;8:207-12.

  24. Li SP, Su ZR, Dong TTX, Tsim KWK. The fruiting body and its caterpillar host of Cordyceps sinensis show close resemblance in main constituents and anti-oxidation activity. Phytomedicine. 2002;9:319-24.

  25. Zhou X, Gong Z, Su Y, Lin J, Tang K. Cordyceps fungi: natural products, pharmacological functions and developmental products. J Pharm Pharmacol. 2009;61:279-91.

  26. Buranov AU, Mazza G. 2010. Extraction and characterization of hemicelluloses from flax shives by different methods. Carbohydr Polym. 2010;79:17-25.

  27. Barker SA, Bourne EJ, Stacey M, Whiffen DH. Infra-red spectra of carbohydrates. Part I. Some derivatives of D-glucopy-ranose. J Chem Soc. 1954;0:171-76.

  28. Lim SF, Zheng YM, Zou SW, Chen JP. Characterization of copper adsorption onto an alginate encapsulated magnetic sorbent by a combined FT-IR, XPS, and mathematical modeling study. Environ Sci Technol. 2008;42:2551-56.

  29. Ooi VEC, Liu F. A review of pharmacological activities of mushroom polysaccharides. Int J Med Mushrooms. 1999;1(3): 195-206.

  30. Wang XH, Xu XJ, Zhang LN. Thermally induced conformation transition of triple-helical lentinan in NaCl aqueous solution. J Phys Chem B. 2008;112:10343-51.

  31. Eigler A, Sinha B, Hartmann G, Endres S. Taming TNF: strategies to restrain this proinflammatory cytokine. Immunol Today. 1997;18:487-92.

  32. Xie QW, Whisnant R, Nathan C. Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon y and bacterial lipopolysaccharide. J Exp Med. 1993;177:1779-84.

  33. Adams V, Nehrhoff B, Spate U, Linke A, Schulze PC, Baur A, Gielen S, Hambrecht R, Schuler G. Induction of iNOS expression in skeletal muscle by IL-1P and NF-kB activation: an in vitro and in vivo study. Cardiovasc Res. 2002;54:95-104.

  34. Hart PH, Brand C, Carson CF, Riley TV, Prager RH, Finlay-Jones JJ. Terpinen-4-ol, the main component of the essential oil of Melaleuca alternifolia (tea tree oil), suppresses inflammatory mediator production by activated human monocytes. Inflamm Res. 2000;49:619-26.

  35. Juergens UR, Engelen J, Racke K, Stober M, Gillissen A, Vetter H. Inhibitory activity of 1,8-cineole (eucalyptol) on cytokine production in cultured human lymphocytes and monocytes. Pulm Pharmacol Ther. 2004;17:281-87.


Articles with similar content:

Anti-Inflammatory Activity and Bioactive Constituents of Cultivated Fruiting Bodies of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 10
Shu-Jing Wu, Ching-Fu Chen, Chih-Chuang Liaw, Lean-Teik Ng, Ming-Nan Lai
Physiological Responses of Anabaena sp. ISC55 to Crude Oil and Its Potential for Biodegradation
International Journal on Algae, Vol.15, 2013, issue 3
R. A. Khavari-Nejad, Samareh Babaei, N. Soltani, F. Najafi, B. Abbaspanah
In Vitro and In Vivo Antitumoral Activity of Exobiopolymers from the Royal Sun Culinary-Medicinal Mushroom Agaricus brasiliensis (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 9
Vanessa Sovrani, Rosilene Rebeca, Franciely Grose Colodi, Herta Stutz Dalla Santa, Tânia Toyomi Tominaga, Mariana de Paula Drewinski, Juliana da Rosa
Immunomodulatory and Antitumor Activities of Water-Soluble Proteoglycan Isolated from the Fruiting Bodies of Culinary-Medicinal Oyster Mushroom Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.9, 2007, issue 2
Sujit Kumar Bhutia, Itisam Sarangi, Dipanjan Ghosh, Indranil Banerje, Saurabh Shah, Swatilekha Maiti, Tapas K Maiti, Sanjaya Kumar Mallick
Preventive Effect of Small Molecular Fraction of Irpex lacteus (Agaricomycetes) Fruiting Body against Chronic Nephritis in Mice and Identification of Active Compounds
International Journal of Medicinal Mushrooms, Vol.22, 2020, issue 4
Yu Han, Haiying Bao, Tolgor Bau, Yu Li, Lie Ma