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ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344
Indexed in
Cloning and Characterization of Polyphenoloxidase DNA from Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae)
Краткое описание
Polyphenoloxidases (PPOs), including tyrosinases and laccases, are ubiquitous metalloenzymes, which have several copper-binding sites. The enzymes cause undesirable browning in vegetables, mushrooms, and fruits, and melanin production of animals. Agaricus brasiliensis is an edible mushroom whose water extracts show biological response modifier effects. Since cold-water extracts of the mushroom fruiting body turn brown on incubation, it has been speculated that the mushroom contains significant amounts of PPOs. Our group has already shown that cold-water extracts indicated PPO activity. Therefore, we cloned a cDNA coding for a PPO from A. brasiliensis cultivated outside in the field. First, a partial sequence of the PPO gene was amplified by reverse transcription (RT)-PCR from the total RNA isolated from A. brasiliensis. Since two copper-binding motifs are well conserved among the PPO amino acid sequences from fungi, two degenerate primers used for the RT-PCR were designed and synthesized based on these sequences. The nucleotide sequence of the amplified fragment showed 83% identity with the corresponding sequence of the A. bisporus PPO gene. Full-length cDNA of the PPO gene was amplified from a cDNA library of A. brasiliensis. Obtained DNA sequences indicated that the amplified cDNA contained an open reading frame of 1731 nucleotides that encoded 576 amino acid residues. Homology search analysis showed that the deduced amino acid sequence was most similar to that of A. bisporus PPO, with 75% identity. The A. brasiliensisfull-length PPO gene was also amplified from the genomic DNA and then se-quenced. Five introns were found within the gene.
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Yamanaka Daisuke, Tada Rui, Adachi Yoshiyuki, Ishibashi Ken-ichi, Motoi Masuro, Iwakura Yoichiro, Ohno Naohito, Agaricus brasiliensis-derived β-glucans exert immunoenhancing effects via a dectin-1-dependent pathway, International Immunopharmacology, 14, 3, 2012. Crossref
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Yamanaka Daisuke, Tamiya Yumi, Motoi Masuro, Ishibashi Ken-ichi, Miura Noriko N., Adachi Yoshiyuki, Ohno Naohito, Heimesaat Markus M., The Effect of Enzymatically Polymerised Polyphenols on CD4 Binding and Cytokine Production in Murine Splenocytes, PLoS ONE, 7, 4, 2012. Crossref
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Hamuro Yuhi, Tajima Katsuya, Matsumoto-Akanuma Akiko, Sakamoto Sayaka, Furukawa Ryutaro, Yamagishi Akihiko, Ohno Naohito, Akanuma Satoshi, Characterization of a thermostable mutant of Agaricus brasiliensis laccase created by phylogeny-based design, Journal of Bioscience and Bioengineering, 124, 6, 2017. Crossref
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Foulongne-Oriol Marie, Rocha de Brito Manuela, Cabannes Delphine, Clément Aurélien, Spataro Cathy, Moinard Magalie, Dias Eustáquio Souza, Callac Philippe, Savoie Jean-Michel, The Genetic Linkage Map of the Medicinal Mushroom Agaricus subrufescens Reveals Highly Conserved Macrosynteny with the Congeneric Species Agaricus bisporus , G3 Genes|Genomes|Genetics, 6, 5, 2016. Crossref