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ISSN Печать: 2155-014X
ISSN Онлайн: 2155-0158
Effect of L-arginine and Corvitin on Oxidative-Nitrosative Stress in Skin of Rats Exposed to Excessive Sodium Nitrate
Краткое описание
The effect of combined action of L-arginine and water soluble form of quercetin (corvitin) on the markers of oxidative-nitrosative stress in rat skin under 30-day intoxication with sodium nitrate (200 mg/kg per day) was investigated. Combined intraperitoneal administration of L-arginine (500 mg/kg) and corvitin (10 mg/kg, based on quercetin), once every 3 days, since the 15th day of intoxication, reduced peroxynitrite generation and superoxide anion radical (·O2-) by NADPH-dependent (endoplasmic reticulum and NO-synthase) and NADH-dependent (mitochondria) electronic transport chains, decreased the concentration of compounds reacting with thiobarbituric acid during incubation in a prooxidant iron-ascorbate buffer solution. The values of these indices were significantly lower compared to the corresponding data obtained by separate administration of these agents. The combined effect of L-arginine and quercetin in the experimental conditions increased the activity of arginase and superoxide dismutase in skin tissues, which significantly exceeded the results of the series with the appointment of only one of these compounds. It has been concluded that the combined administration of L-arginine and corvitin is more effective for preventing oxidative-nitrosative stress in the skin of rats exposed to excessive nutrient intake of sodium nitrate compared with their separate application, it decreases the formation of active forms of oxygen and nitrogen in the skin tissues (·O2- and peroxynitrite), enhancing antioxidant potential, arginase and superoxide dismutase activities.