Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Critical Reviews™ in Eukaryotic Gene Expression
Импакт фактор: 2.156 5-летний Импакт фактор: 2.255 SJR: 0.649 SNIP: 0.599 CiteScore™: 3

ISSN Печать: 1045-4403
ISSN Онлайн: 2162-6502

Выпуски:
Том 30, 2020 Том 29, 2019 Том 28, 2018 Том 27, 2017 Том 26, 2016 Том 25, 2015 Том 24, 2014 Том 23, 2013 Том 22, 2012 Том 21, 2011 Том 20, 2010 Том 19, 2009 Том 18, 2008 Том 17, 2007 Том 16, 2006 Том 15, 2005 Том 14, 2004 Том 13, 2003 Том 12, 2002 Том 11, 2001 Том 10, 2000 Том 9, 1999 Том 8, 1998 Том 7, 1997 Том 6, 1996 Том 5, 1995 Том 4, 1994

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukarGeneExpr.v9.i3-4.200
pages 353-361

DNA Replication and Nuclear Organization: Prospects for a Soluble In Vitro System

Daniela S. Dimitrova
Department of Biochemistry and Molecular Biology, S.U.N.Y. Health Science Center, 750 East Adams Street, Syracuse, NY 13210
David M. Gilbert
Department of Biochemistry and Molecular Biology, S.U.N.Y. Health Science Center, 750 East Adams Street, Syracuse, NY 13210

Краткое описание

The role of nuclear structure in the replication of eukaryotic DNA has been the subject of debate for many decades. The recent demonstration that once-per-cell-cycle replication can take place in vitro without a nucleus, providing sufficiently high concentrations of replication factors are supplied, suggests that one role of the nucleus is to concentrate essential factors. This important finding has paved the way for the establishment of a purified biochemical system for replication of eukaryotic DNA. However, this soluble system, derived from Xenopus egg extracts, initiates replication within any DNA sequence and does not recapitulate the spatial and temporal regulation of DNA replication that is observed in most cells. In both Xenopus and Drosophila embryos, site-specific initiation of replication is not observed until after nuclei become transcriptionally active at the blastula stage of development. Furthermore, programmed changes in both the locations of origins and the time during S-phase at which sequences are replicated accompany key stages of metazoan development. Recent findings indicate that these changes correlate with changes in nuclear organization and that the spatial and temporal program for replication is established early in Gl-phase when nuclei are structurally and functionally reorganized after mitosis.


Articles with similar content:

Interrelationships between Nuclear Structure and Ligand-Activated Intracellular Receptors
Critical Reviews™ in Eukaryotic Gene Expression, Vol.6, 1996, issue 2-3
Thomas S. Ruh, Mary F. Ruh, Robert Dunn, II
Complex Smad-Dependent Transcriptional Responses in Vertebrate Development and Human Disease
Critical Reviews™ in Eukaryotic Gene Expression, Vol.12, 2002, issue 2
Leo A. van Grunsven, Danny Huylebroeck, Kristin Verschueren
The Roles of Chromatin Remodeling Genes in Pancreatic-Biliary Malignancies
Critical Reviews™ in Oncogenesis, Vol.22, 2017, issue 5-6
Claudio Luchini, Alessia Nottegar
On the Relationship of Matrix Association and DNA Replication
Critical Reviews™ in Eukaryotic Gene Expression, Vol.10, 2000, issue 1
Michael J. Schell, Bruna P. Brylawski, Stephanie M. Cohen, Mania Cordeiro-Stone, David G. Kaufman
Combinatorial Transcriptional Regulation: The Interaction of Transcription Factors and Cell Signaling Molecules with Homeodomain Proteins in Drosophila Development
Critical Reviews™ in Eukaryotic Gene Expression, Vol.11, 2001, issue 1-3
Sarah E. Bondos, Xin-Xing Tan