Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Critical Reviews™ in Eukaryotic Gene Expression
Импакт фактор: 2.156 5-летний Импакт фактор: 2.255 SJR: 0.649 SNIP: 0.599 CiteScore™: 3

ISSN Печать: 1045-4403
ISSN Онлайн: 2162-6502

Выпуски:
Том 30, 2020 Том 29, 2019 Том 28, 2018 Том 27, 2017 Том 26, 2016 Том 25, 2015 Том 24, 2014 Том 23, 2013 Том 22, 2012 Том 21, 2011 Том 20, 2010 Том 19, 2009 Том 18, 2008 Том 17, 2007 Том 16, 2006 Том 15, 2005 Том 14, 2004 Том 13, 2003 Том 12, 2002 Том 11, 2001 Том 10, 2000 Том 9, 1999 Том 8, 1998 Том 7, 1997 Том 6, 1996 Том 5, 1995 Том 4, 1994

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukaryotGeneExpr.2013007558
pages 1-13

Suspension Culture of Pluripotent Stem Cells: Effect of Shear on Stem Cell Fate

Kevin C. Keller
Department of Cell Biology and Neuroscience and Stem Cell Center, College of Natural and Agricultural Sciences, University of California Riverside, Riverside, CA 92521
Beatriz Rodrigues
Department of Cell Biology and Neuroscience and Stem Cell Center, College of Natural and Agricultural Sciences, University of California Riverside, Riverside, CA 92521
Nicole I. zur Nieden
Department of Cell Biology and Neuroscience and Stem Cell Center, College of Natural and Agricultural Sciences, University of California Riverside, Riverside, CA 92521

Краткое описание

Despite significant promise, the routine usage of suspension cell culture to manufacture stem cell−derived differentiated cells has progressed slowly. Suspension culture is an innovative way of either expanding or differentiating cells and sometimes both are combined into a single bioprocess. Its advantages over static 2D culturing include a homogeneous and controllable culture environment and producing a large quantity of cells in a fraction of time. This feature makes suspension cell culture ideal for use in stem cell research and eventually ideal in the large-scale production of differentiated cells for regenerative medicine. Because of their tremendous differentiation capacities and unlimited growth properties, pluripotent stem cells (PSCs) in particular are considered potential sources for future cell-replacement therapies. Currently, expansion of PSCs is accomplished in 2D, which only permits a limited amount of cell growth per culture flask before cells need to be passaged. However, before stem cells can be applied clinically, several aspects of their expansion, such as directed growth, but also differentiation, need to be better controlled. This review will summarize recent advantages in suspension culture of PSCs, while at the same time highlighting current challenges.


Articles with similar content:

The Ethics of Gene Editing Technologies in Human Stem Cells
Ethics in Biology, Engineering and Medicine: An International Journal, Vol.5, 2014, issue 4
Richard L. Wilson, Elena Artimovich, Michael W. Nestor
A New Perspective for Stem-Cell Mechanobiology: Biomechanical Control of Stem-Cell Behavior and Fate
Critical Reviews™ in Biomedical Engineering, Vol.38, 2010, issue 5
Michael Cho, Jennifer Shin, Igor A. Titushkin
Bacteria-Containing Vacuoles: Subversion of Cellular Membrane Traffic and Autophagy
Critical Reviews™ in Eukaryotic Gene Expression, Vol.25, 2015, issue 2
Bor Luen Tang
Toward Prediction of Immune Mechanisms and Design of Immunotherapies in Melanoma
Critical Reviews™ in Biomedical Engineering, Vol.40, 2012, issue 4
Frank O. Nestle, Louise Saul, Sophia N. Karagiannis, Sophia Tsoka, Debra H. Josephs, Chrysanthi Ainali, Panagiotis Karagiannis
Potential Role of Induced Pluripotent Stem Cells as Regenerative Medicine in Retinal Cell Damage
Journal of Environmental Pathology, Toxicology and Oncology, Vol.37, 2018, issue 4
Yi-Ran Pan, Ying-Jian Sun, Bin Fan, Guang-Yu Li