Доступ предоставлен для: Guest
Plasma Medicine

Выходит 4 номеров в год

ISSN Печать: 1947-5764

ISSN Онлайн: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

Indexed in

Comparison of Biological Effects on Human Keratinocytes Using Different Plasma Treatment Regimes

Том 3, Выпуск 1-2, 2013, pp. 57-69
DOI: 10.1615/PlasmaMed.2014008219
Get accessDownload

Краткое описание

This study investigated the influence of the plasma treatment regime on human keratinocytes (HaCaT cells). HaCaT cells were plasma treated with a volume dielectric barrier discharge plasma source in three different ways: directly, directly with culture medium exchange, and indirectly (in which only cell culture medium was exposed to plasma). The influence of dielectric barrier discharge plasma on viability, DNA, cell cycle, and intracellular concentration of reactive oxygen species in HaCaT cells was investigated. Direct and indirect plasma treatment caused a treatment time−dependent decrease of viable cells. An increase in DNA damage was observed immediately after plasma treatment, which was diminished after 24 h. Intracellular reactive oxygen species increased with longer plasma treatment times. The cell cycle analysis showed an accumulation of cells in the G2/M phase at the expense of cells in the G1 phase. An immediate exchange of culture medium after plasma treatment attenuated the described effects. Direct and indirect plasma treatment of adherent HaCaT cells resulted in comparable effects that depend on the plasma treatment time. Physical plasma seems to generate long-living reactive species or to modify organic components of the cell culture medium. Both mechanisms can initiate oxidative stress in human keratinocytes, which is responsible for the observed effects.

ЦИТИРОВАНО В
  1. Haertel Beate, Woedtke Thomas von, Weltmann Klaus-Dieter, Lindequist Ulrike, Non-Thermal Atmospheric-Pressure Plasma Possible Application in Wound Healing, Biomolecules & Therapeutics, 22, 6, 2014. Crossref

  2. Korolov Ihor, Fazekas Barbara, Széll Márta, Kemény Lajos, Kutasi Kinga, The effect of the plasma needle on the human keratinocytes related to the wound healing process, Journal of Physics D: Applied Physics, 49, 3, 2016. Crossref

  3. Hänsch Mareike A C, Mann Miriam, Weltmann Klaus-Dieter, von Woedtke Thomas, Analysis of antibacterial efficacy of plasma-treated sodium chloride solutions, Journal of Physics D: Applied Physics, 48, 45, 2015. Crossref

  4. Girard Pierre-Marie, Arbabian Atousa, Fleury Michel, Bauville Gérard, Puech Vincent, Dutreix Marie, Sousa João Santos, Synergistic Effect of H2O2 and NO2 in Cell Death Induced by Cold Atmospheric He Plasma, Scientific Reports, 6, 1, 2016. Crossref

  5. Zhang Jiao, Kwon Taeho, Kim Seong, Jeong Dong, Plasma Farming: Non-Thermal Dielectric Barrier Discharge Plasma Technology for Improving the Growth of Soybean Sprouts and Chickens, Plasma, 1, 2, 2018. Crossref

  6. Privat-Maldonado Angela, Schmidt Anke, Lin Abraham, Weltmann Klaus-Dieter, Wende Kristian, Bogaerts Annemie, Bekeschus Sander, ROS from Physical Plasmas: Redox Chemistry for Biomedical Therapy, Oxidative Medicine and Cellular Longevity, 2019, 2019. Crossref

  7. Dzimitrowicz Anna, Bielawska-Pohl Aleksandra, Jamroz Piotr, Dora Jerzy, Krawczenko Agnieszka, Busco Giovanni, Grillon Catherine, Kieda Claudine, Klimczak Aleksandra, Terefinko Dominik, Baszczynska Aleksandra, Pohl Pawel, Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature, Plasma Chemistry and Plasma Processing, 40, 1, 2020. Crossref

  8. Haralambiev Lyubomir , Wien Lasse , Gelbrich Nadine , Lange J�rn , Bakir Sinan , Kramer Axel , Burchardt Martin , Ekkernkamp Axel , G�mbel Denis , Stope Matthias , Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used, Oncology Letters, 2019. Crossref

  9. Sklias Kyriakos, Santos Sousa João, Girard Pierre-Marie, Role of Short- and Long-Lived Reactive Species on the Selectivity and Anti-Cancer Action of Plasma Treatment In Vitro, Cancers, 13, 4, 2021. Crossref

  10. Bekeschus Sander, Schmidt Anke, Weltmann Klaus-Dieter, von Woedtke Thomas, The plasma jet kINPen – A powerful tool for wound healing, Clinical Plasma Medicine, 4, 1, 2016. Crossref

Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции Цены и условия подписки Begell House Контакты Language English 中文 Русский Português German French Spain