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Critical Reviews™ in Biomedical Engineering

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ISSN Печать: 0278-940X

ISSN Онлайн: 1943-619X

SJR: 0.262 SNIP: 0.372 CiteScore™:: 2.2 H-Index: 56

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Recent Advances in Optical Microscopy Methods for Subcellular Imaging of Thick Biological Tissues

Том 41, Выпуск 4-5, 2013, pp. 393-403
DOI: 10.1615/CritRevBiomedEng.2014010461
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Краткое описание

Optical microscopy has been widely applied in cellular and subcellular imaging. Conventional light microscopes, however, have rather limited imaging depth and are limited to imaging only mechanically sectioned thin samples. Multiphoton microscopy and optical coherence microscopy are common techniques for diffraction-limited imaging beyond an imaging depth of 0.5 mm. Focal modulation microscopy is a novel method that combines confocal spatial filtering with focal modulation to reject out-of-focus backgrounds. Focal modulation microscopy has demonstrated an imaging depth comparable to those of multiphoton microscopy and optical coherence microscopy, near-real-time image acquisition, and capability with a multiple contrast mechanism.

Ключевые слова: Microscopy, imaging depth, scattering, fluorescence, contrast
ЦИТИРОВАНО В
  1. Pant Shilpa, Li Caixia, Gong Zhiyuan, Chen Nanguang, Line-scan focal modulation microscopy, Journal of Biomedical Optics, 22, 05, 2017. Crossref

  2. Venkateshaiah Abhilash, Padil Vinod V.T., Nagalakshmaiah Malladi, Waclawek Stanisław, Černík Miroslav, Varma Rajender S., Microscopic Techniques for the Analysis of Micro and Nanostructures of Biopolymers and Their Derivatives, Polymers, 12, 3, 2020. Crossref

  3. Romanov Andrey V., Yurkin Maxim A., Single‐Particle Characterization by Elastic Light Scattering, Laser & Photonics Reviews, 15, 2, 2021. Crossref

  4. Hahn Artur, Kurz Felix T., Advanced imaging/MRI for tissue engineering, in Tissue Engineering Using Ceramics and Polymers, 2022. Crossref

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