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Hydrobiological Journal
SJR: 0.227 SNIP: 0.575 CiteScore™: 0.24

ISSN Печать: 0018-8166
ISSN Онлайн: 1943-5991

Выпуски:
Том 55, 2019 Том 54, 2018 Том 53, 2017 Том 52, 2016 Том 51, 2015 Том 50, 2014 Том 49, 2013 Том 48, 2012 Том 47, 2011 Том 46, 2010 Том 45, 2009 Том 44, 2008 Том 43, 2007 Том 42, 2006 Том 41, 2005 Том 40, 2004 Том 39, 2003 Том 38, 2002 Том 37, 2001 Том 36, 2000 Том 35, 1999 Том 34, 1998

Hydrobiological Journal

DOI: 10.1615/HydrobJ.v48.i5.100
pages 107-112

Method of Intravital Morphometry of the Nucleated Erythrocytes of Fishes

A.Yu. Andreyeva
Institute of Biology of South Seas National Academy of Sciences of Ukraine Sevastopol, Ukraine
V. S. Mukhanov
A.O. Kovalevsky Institute of Biology of the Southern Seas, 2, Nakhimov Prosp., Sevastopol 99011, Crimea

Краткое описание

The method of intravital morphology of fish erythrocytes using vital stains has been developed. For the nuclei staining fluorochrome SYBR Green 1 was used. The photos of erythrocytes in thick drop were made; pictures were analyzed by the ImageJ 1.44p software. Obtained values of length of the large and small axes of nuclei and cells were averaged and compared with values, measured on the blood smears stained according to the Pappenheim method. Differences did not exceed 2%.


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