Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Journal of Long-Term Effects of Medical Implants
SJR: 0.145 SNIP: 0.491 CiteScore™: 0.89

ISSN Печать: 1050-6934
ISSN Онлайн: 1940-4379

Выпуски:
Том 30, 2020 Том 29, 2019 Том 28, 2018 Том 27, 2017 Том 26, 2016 Том 25, 2015 Том 24, 2014 Том 23, 2013 Том 22, 2012 Том 21, 2011 Том 20, 2010 Том 19, 2009 Том 18, 2008 Том 17, 2007 Том 16, 2006 Том 15, 2005 Том 14, 2004 Том 13, 2003 Том 12, 2002 Том 11, 2001 Том 10, 2000

Journal of Long-Term Effects of Medical Implants

DOI: 10.1615/JLongTermEffMedImplants.2015011695
pages 147-160

Marrow Stem Cell Differentiation for Valvulogenesis via Oscillatory Flow and Nicotine Agonists: Unusual Suspects?

Smita Bhatacharjee
Tissue Engineered Mechanics, Imaging and Materials Laboratory (TEMIM Lab), Department of Biomedical Engineering, Florida International University, Miami, Florida
Sasmita Rath
Manuel Salinas
Tissue Engineered Mechanics, Imaging and Materials Laboratory (TEMIM Lab), Department of Biomedical Engineering, Florida International University, Miami, Florida
Sharan Ramaswamy
Department of Biomedical Engineering, Florida International University Miami, Florida, USA

Краткое описание

Fluid-induced oscillatory shear stress (OSS) and nicotine are known antagonists in cardiovascular disease. However, from a regenerative medicine standpoint, we hypothesized that these parameters may support the cell differentiation of bone marrow mesenchymal stem cells (BMMSCs) for engineering heart valves. In this study, OSS and nicotine (10-6M) were applied individually to BMMSCs in monolayer culture. In both cases, a significantly higher expression of CD31 was detected compared to corresponding controls (p<0.05). We interpret our findings to indicate that both OSS and nicotine independently support mesenchymal to endothelial transformation; however, the underlying mechanism for this transformation in terms of the cell cytoskeletal structure was entirely different between the two stimulants. In the case of OSS, F-actin filaments exhibited a stretching response and formed a preferential alignment with each other. However, in the nicotine-treated group, a clear increase was observed in the number of actin filaments present, which led to the maximum expression of CD31 in comparison to the OSS and control groups. From our findings, we speculate that while nicotine may stimulate an increase in the differentiation of BMMSCs to endothelial cells, OSS may play a greater role in cellular distribution and the eventual creation of a tissue engineered heart valve (TEHV) endothelium.


Articles with similar content:

Total Knee Arthroplasty after Complete Patellectomy: A Review
Journal of Long-Term Effects of Medical Implants, Vol.25, 2015, issue 3
Anthony J. Scillia, Sargoun Issa, Anthony Festa, Michael A. Mont, Kimona Issa, D. Alex Stroh
Retinal Pigment Epithelium Differentiation of Stem Cells: Current Status and Challenges
Critical Reviews™ in Biomedical Engineering, Vol.37, 2009, issue 4-5
Martin Yarmush, Basak E. Uygun, Nripen Sharma
Culture of Human A375 Melanoma Cells in the Presence of Fibronectin Causes Expression of MMP-9 and Activation of MMP-2 in Culture Supernatants
Journal of Environmental Pathology, Toxicology and Oncology, Vol.27, 2008, issue 2
Shamik Das, Amitava Chatterjee, Aniruddha Banerji
CONDITIONAL EQUITY METRICS AS TOOLS FOR EVALUATING EQUITY IN SCHOOLS AND EDUCATION SYSTEMS
Journal of Women and Minorities in Science and Engineering, Vol.4, 1998, issue 2&3
Norman L. Webb
Reflections on Lineage Potential of Skeletal Muscle Satellite Cells: Do They Sometimes Go MAD?
Critical Reviews™ in Eukaryotic Gene Expression, Vol.17, 2007, issue 1
Zipora Yablonka-Reuveni, Gabi Shefer