Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Journal of Environmental Pathology, Toxicology and Oncology
Импакт фактор: 1.625 5-летний Импакт фактор: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Печать: 0731-8898
ISSN Онлайн: 2162-6537

Выпуски:
Том 39, 2020 Том 38, 2019 Том 37, 2018 Том 36, 2017 Том 35, 2016 Том 34, 2015 Том 33, 2014 Том 32, 2013 Том 31, 2012 Том 30, 2011 Том 29, 2010 Том 28, 2009 Том 27, 2008 Том 26, 2007 Том 25, 2006 Том 24, 2005 Том 23, 2004 Том 22, 2003 Том 21, 2002 Том 20, 2001

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v26.i3.20
pages 173-183

Heat Shock Protein 27 Protects against Aminolevulinic Acid-Mediated Photodynamic Therapy-Induced Apoptosis and Necrosis in Human Breast Cancer Cells

Sarah A. Ziegler
Department. of Chemistry, University of Nevada Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154-4003, USA
Cherisse Loucks
Department. of Chemistry, University of Nevada Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154-4003, USA
Steen J. Madsen
Department of Health Physics and Diagnostic Sciences, University of Nevada, Las Vegas, 4505 S. Maryland Pkwy., Box 453037, Las Vegas, NV 89154
Stephen W. Carper
Department. of Chemistry, University of Nevada Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154-4003; and Department of Health Physics, University of Nevada Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154-3037, USA

Краткое описание

This study utilized two breast cancer cell lines differing only in their expression of heat shock protein 27 (hsp27). The DB46 cell line was engineered to express high constitutive levels of hsp27, while the DC4 cell line expresses normal low levels of hsp27. The cells were incubated in 1 mM aminolevlinic acid (ALA) 4 hr prior to light exposures (635 nm) ranging from 1 to 20 J/cm2. Both cell lines displayed a dose response to photodynamic therapy (PDT) as assayed by clonogenic survival. LD50s of 2.68 and 1.27 J/cm2 were observed for DB46 and DC4 cells respectively. ALA-PDT-induced resistance to both apoptosis and necrosis in the DB46 cell line was found from TUNEL assays and fluorescence microscopy studies using propidium iodide and Hoechst staining.


Articles with similar content:

Photothermal Therapy Employing Gold Nanoparticle- Loaded Macrophages as Delivery Vehicles: Comparing the Efficiency of Nanoshells Versus Nanorods
Journal of Environmental Pathology, Toxicology and Oncology, Vol.36, 2017, issue 3
Henry Hirschberg, Catherine Christie, Steen J. Madsen, Qian Peng
Vernolide-A Inhibits Radiation-Induced Hypoxia-Mediated Tumor Angiogenesis by Regulating HIF-1α, MMP-2, MMP-9, and VEGF
Journal of Environmental Pathology, Toxicology and Oncology, Vol.30, 2011, issue 2
P. Pratheeshkumar, Girija Kuttan
Immunohistochemical Study of Fibrosis and Adenocarcinoma in Dominant-Negative p53 Transgenic Mice Exposed to Chrysotile Asbestos and Benzo(a)pyrene
Journal of Environmental Pathology, Toxicology and Oncology, Vol.27, 2008, issue 4
William N. Rom, Ting-An Yie, Judith D. Goldberg, Herman T. Yee, Kam Meng Tchou Wong
The Shaggy Ink Cap Medicinal Mushroom, Coprinus comatus (Higher Basidiomycetes) Extract Induces Apoptosis in Ovarian Cancer Cells via Extrinsic and Intrinsic Apoptotic Pathways
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 12
Solomon P. Wasser, Amal Rouhana-Toubi, Fuad Fares
Identification of Potential Oncogenic Long Non-Coding RNA Set as a Biomarker Associated with Colon Cancer Prognosis
Journal of Environmental Pathology, Toxicology and Oncology, Vol.39, 2020, issue 1
Jialin Yang, Ming Fan, Meihua Chen, Jinyi Lang