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Journal of Environmental Pathology, Toxicology and Oncology
Импакт фактор: 1.625 5-летний Импакт фактор: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Печать: 0731-8898
ISSN Онлайн: 2162-6537

Выпуски:
Том 39, 2020 Том 38, 2019 Том 37, 2018 Том 36, 2017 Том 35, 2016 Том 34, 2015 Том 33, 2014 Том 32, 2013 Том 31, 2012 Том 30, 2011 Том 29, 2010 Том 28, 2009 Том 27, 2008 Том 26, 2007 Том 25, 2006 Том 24, 2005 Том 23, 2004 Том 22, 2003 Том 21, 2002 Том 20, 2001

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvPathToxOncol.v23.i1.30
12 pages

Radiation-Induced DNA Damage: Formation, Measurement, and Biochemical Features

Jean Cadet
Laboratoire "Lesions des Acides Nucleiques," SCIB/DRFMC, CEA/Grenoble, France
Sophie Bellon
Laboratoire "Lesions des Acides Nucleiques," SCIB/DRFMC, CEA/Grenoble, France
Thierry Douki
Laboratoire "Lesions des Acides Nucleiques," SCIB/DRFMC, CEA/Grenoble, France
Sandrine Frelon
Laboratoire "Lesions des Acides Nucleiques," SCIB/DRFMC, CEA/Grenoble, France
Didier Gasparutto
Laboratoire "Lesions des Acides Nucleiques," SCIB/DRFMC, CEA/Grenoble, France
Evelyne Muller
Laboratoire "Lesions des Acides Nucleiques," SCIB/DRFMC, CEA/Grenoble, France
Jean-Pierre Pouget
Laboratoire "Lesions des Acides Nucleiques," SCIB/DRFMC, CEA/Grenoble, France
Jean-Luc Ravanat
Laboratoire "Lesions des Acides Nucleiques," SCIB/DRFMC, CEA/Grenoble, France
Anthony Romieu
Laboratoire "Lesions des Acides Nucleiques," SCIB/DRFMC, CEA/Grenoble, France
Sylvie Sauvaigo
Laboratoire "Lesions des Acides Nucleiques," SCIB/DRFMC, CEA/Grenoble, France

Краткое описание

Most of the reactions induced by ·OH radicals (indirect effects) and by one-electron oxidation (direct effects) as the result of exposure to ionizing radiation may be described for the four main DNA nucleobases. Relevant information is now available on the formation of single and tandem base lesions implicating guanine as the most susceptible DNA component to the deleterious effects of ionizing radiation. In contrast, there is still a paucity of information on the radiation-induced formation of base damage within cellular DNA. This is mostly a result of difficulties associated with the measurement of oxidized purine and pyrimidine bases that appear to be generated in very low yields. This is illustrated by the measurement of low amounts of E. coli formamidopyrimidine glycosylase- and endonuclease-III-sensitive sites in the DNA of neoplastic monocytes upon exposure to gamma rays (48 and 53 per 109 bases and per Gy, respectively) using a modified comet assay (the overall number of strand breaks and alkali-labile sites was estimated to be 130 per 109 bases and per Gy). More specifically, the level of several radiation-induced modified bases, including thymine glycols, 5-formyluracil, 5-(hydroxymethyl)uracil, 8-oxo-7,8-dihydroguanine, and 8-oxo-7,8-dihydroadenine, together with related formamidopyrimidine derivatives was assessed using the suitable HPLC-MS/MS method. Information is also provided on the substrate specificity of DNA repair enzymes and the mutagenic potential of base lesions using site-specific modified oligonucleotides as the probes.


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