Доступ предоставлен для: Guest
Портал Begell Электронная Бибилиотека e-Книги Журналы Справочники и Сборники статей Коллекции
Journal of Environmental Pathology, Toxicology and Oncology
Импакт фактор: 1.625 5-летний Импакт фактор: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN Печать: 0731-8898
ISSN Онлайн: 2162-6537

Выпуски:
Том 39, 2020 Том 38, 2019 Том 37, 2018 Том 36, 2017 Том 35, 2016 Том 34, 2015 Том 33, 2014 Том 32, 2013 Том 31, 2012 Том 30, 2011 Том 29, 2010 Том 28, 2009 Том 27, 2008 Том 26, 2007 Том 25, 2006 Том 24, 2005 Том 23, 2004 Том 22, 2003 Том 21, 2002 Том 20, 2001

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvPathToxOncol.v23.i1.70
14 pages

p53 Binding to Target Sites Is Dynamically Regulated Before and After Ionizing Radiation-Mediated DNA Damage

Meredith E. Crosby
Department of Cancer Biology, Lerner Research Institute and Department of Radiation Oncology, The Cleveland Clinic Foundation; and Department of Environmental Health Sciences, Case Western Reserve School of Medicine, Cleveland, Ohio, USA
Marcela Oancea
Department of Cancer Biology, Lerner Research Institute and Department of Radiation Oncology, The Cleveland Clinic Foundation; and Department of Chemistry, Cleveland State University, Cleveland, Ohio, USA
Alex Almasan
Dept.of Cancer Biology,Lerner Research Institute and Dept.of Radiation Oncology,The Cleveland Clinic Foundation; Dept.of Environmental Health Sciences,Case Western Reserve School of Medicine; Dept.of Chemistry,Cleveland State University, Cleveland,OH,USA

Краткое описание

Although radiation therapy has been an important modality for cancer treatment, the molecular mechanisms underlying the overall genomic response of mammalian cells to radiation are not well characterized. The success of radiation therapy using ionizing radiation relies upon the regulation of both the cell cycle and apoptosis, as conferred by the activation of DNA damage-responsive genes. To better understand the key players involved in this response, expression-profiling experiments were performed using custom-made cDNA microarrays. In MOLT-4 lymphoma tumor cells, the induction of target gene products following irradiation supports a major role for p53 as a transcriptional activator, but also invokes questions regarding conditional transcription regulation following irradiation. Using chromatin immunoprecipitation (ChIP), p53 binding to chromatin was examined following irradiation using primers that are specific for p53 binding sites in target genes. PCR analysis indicates dynamic target gene binding.Thus, at 8 hours following radiation treatment, the p21 and puma promoter sites were characterized by relative increases in chromatin precipitation, while the bax promoter site was not. Because the binding of p53 to these sites only changed modestly following radiation, other studies were conducted to characterize the presence of constitutive binding to putative p53 DNA binding sites in several other genes.


Articles with similar content:

Stable Water Clusters−Mediated Molecular Alterations in Human Melanoma Cell Lines
Forum on Immunopathological Diseases and Therapeutics, Vol.3, 2012, issue 3-4
Stavroula Baritaki, Benjamin Bonavida
Localization of the Werner Protein Together with H2AX in γ-Irradiation-Induced Neoplastic Transformed Human Mesenchymal Stem Cells
Critical Reviews™ in Eukaryotic Gene Expression, Vol.27, 2017, issue 1
Tufan Cankaya, Nedime Serakinci, Steen Kølvraa
2Am-DNT Induces Cell Death and Apoptosis in Human Cells
Journal of Environmental Pathology, Toxicology and Oncology, Vol.28, 2009, issue 3
Z. Hawkins, M. Asktorab, M. Yakubu, S. K. Dutta, D. Smoot, Hirendranath Banerjee
Cyclins, Cyclin-Dependent Kinases and Cdk Inhibitors: Implications in Cell Cycle Control and Cancer
Critical Reviews™ in Eukaryotic Gene Expression, Vol.5, 1995, issue 2
Antonio Giordano, Timothy K. MacLachlan, Nianli Sang
Apoptotic Effect of Extract from Medicinal Mushroom from Taiwan Taiwanofungus salmoneus (Higher Basidiomycetes) Mycelium Combined with or without Cisplatin on Hepatocellular Carcinoma Cells
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 6
Yun-Jung Hsieh, Jeng-Leun Mau, Rao-Chi Chien