Выходит 4 номеров в год
ISSN Печать: 0731-8898
ISSN Онлайн: 2162-6537
Indexed in
Histochemical, Ultrastructural, and Biochemical Evidences for Azadirachta indica−Induced Apoptosis in Benzo(a)pyrene−Induced Murine Forestomach Tumors
Краткое описание
The present study reports aqueous Azadirachta indica leaf extract (AAILE)−mediated induction of apoptosis in a murine forestomach tumorigenesis model. Histochemistry-based quantification of apoptosis revealed enhanced apoptotic index in the forestomach tumors of mice receiving AAILE along with benzo(a)pyrene (B(a)P). Transmission electron microscopy confirmed the presence of classical morphological features of apoptosis including chromatin condensation/marginalization, nuclear fragmentation, and formation of apoptotic bodies. Scanning electron microscopy showed surface modifications on the transformed squamous epithelial cells and certain mitotic cells among them over the forestomach tumors of mice receiving only B(a)P. In tumors of the mice receiving AAILE along with B (a)P, such mitotic cells were found to be absent; however, certain cells showing shrinkage and blebbings (characteristics of apoptosis) were observed. DNA fragmentation was observed to increase exclusively in the tumors of mice that received AAILE along with B(a)P. Lipid peroxidation (LPO) levels decreased in forestomach tissues of mice in all the groups studied when compared to control counterparts. However, levels of LPO were found to increase in the tumorous tissue of mice that received AAILE along with B(a)P when compared to mice receiving only B(a)P. Taken together, observations of the present study suggest that A. indica induces apoptosis in B(a)P-induced murine forestomach tumors.
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Arora N., Bansal M. P., Koul A., Azadirachta indicaacts as a pro-oxidant and modulates cell cycle associated proteins during DMBA/TPA induced skin carcinogenesis in mice, Cell Biochemistry and Function, 31, 5, 2013. Crossref
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Cheng Tao, Zhang Jian-Guo, Cheng Yuan-He, Gao Zhong-Wei, Ren Xiao-Qiang, Relationship Between PTEN and Livin Expression and Malignancy of Renal Cell Carcinomas, Asian Pacific Journal of Cancer Prevention, 13, 6, 2012. Crossref
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Bharati Sanjay, Rishi Praveen, Koul Ashwani, Azadirachta indica Modulates Electrical Properties and Type of Cell Death in NDEA-Induced Hepatic Tumors, Cell Biochemistry and Biophysics, 70, 1, 2014. Crossref
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Arora N., Koul A., Bansal M. P., Chemopreventive activity of Azadirachta indica on two-stage skin carcinogenesis in murine model, Phytotherapy Research, 2010. Crossref
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Bharati Sanjay, Rishi Praveen, Koul Ashwani, Azadirachta indica exhibits chemopreventive action against hepatic cancer: Studies on associated histopathological and ultrastructural changes, Microscopy Research and Technique, 75, 5, 2012. Crossref
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Lim T. K., Azadirachta indica, in Edible Medicinal and Non Medicinal Plants, 2014. Crossref
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He Zhenxiang, Jiang Cuihua, Zhang Jian, Yin Zhiqi, Yin Zengfang, Zhu Yunfeng, Fu Jie, Neem tree (Azadirachta indica) extract specifically suppresses the growth of tumors in H22-bearing Kunming mice, Zeitschrift für Naturforschung C, 71, 7-8, 2016. Crossref
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Chugh N.A., Koul A., Altered presence of extra cellular matrix components in murine skin cancer: Modulation by Azadirachta indica leaf extract, Journal of Traditional and Complementary Medicine, 11, 3, 2021. Crossref
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Abdelmeguid Nabila E., Khalil Mahmoud I., Badr Nada S., Alkhuriji Afrah F., El-Gerbed Mohamed S.A., Sultan Ahmed S., Ameliorative effects of colostrum against DMBA hepatotoxicity in rats, Saudi Journal of Biological Sciences, 28, 4, 2021. Crossref
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salah Ebtihal, El esh Heba, Abdel-Reheim Eman S., Abdul-Hamid Manal, Ameliorative effects of Artemisia and Echinacea extracts against hepato and cardiotoxicity induced by DMBA on albino rats: experimental and molecular docking analyses, Beni-Suef University Journal of Basic and Applied Sciences, 11, 1, 2022. Crossref