RT Journal Article
ID 5b1694f16d976987
A1 Guo, Xuefang
A1 Wang, Xiaoli
A1 Liu, Min
A1 Chen, Lei
A1 Chanda, Warren
A1 Pahiar, Arshad Ahmed
A1 Li, Xingyun
A1 Zhang, Wei
A1 Ning, Anhong
A1 Huang, Min
A1 Cao, Jing
A1 Zhong, Mintao
T1 Expression and Antitumor Function of Latcripin-4 RCC1 and ANK Domain Protein on HepG2 from the Shiitake Medicinal Mushroom, Lentinus edodes C91–3 (Agaricomycetes), Transcriptome
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-11-30
VO 20
IS 12
SP 1163
OP 1172
K1 Lentinus edodes
K1 transcriptome
K1 Latcripin-4 RCC1 and ANK domain protein
K1 antitumor
K1 medicinal mushrooms
AB The recombinant protein of Latcripin-4 regulator of chromosome condensation 1 (RCC1) and ankyrin (ANK) domains were expressed and the antitumor activity of Latcripin-4 on HepG2 cells was studied. First, the Latcripin-4 transcript was selected from the medicinal mushroom Lentinus edodes C91–3 transcriptome by bioinformatics.
Then the full-length gene of Latcripin-4 was isolated with 3'-full rapid amplification of cDNA ends (RACE) and 5'-full RACE methods according to the transcriptome. The RCC1 and ANK domains from the full-length gene were selected and inserted into the expression vector pET-32a (+) and expressed in Escherichia coli Rosetta-gami (DE3). Western blotting indicated that the protein was expressed successfully. The biological function of Latcripin-4 RCC1 and ANK domain protein on HepG2 cells was studied with the CCK-8 assay. All results demonstrated that Latcripin-4 RCC1 and ANK domain protein can inhibit the growth of human HepG2 liver cancer cells, which brings new insights to identifying antitumor proteins from medicinal food for cancer therapy.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2c6ab3993645ca9e,5b1694f16d976987.html