RT Journal Article ID 5b1694f16d976987 A1 Guo, Xuefang A1 Wang, Xiaoli A1 Liu, Min A1 Chen, Lei A1 Chanda, Warren A1 Pahiar, Arshad Ahmed A1 Li, Xingyun A1 Zhang, Wei A1 Ning, Anhong A1 Huang, Min A1 Cao, Jing A1 Zhong, Mintao T1 Expression and Antitumor Function of Latcripin-4 RCC1 and ANK Domain Protein on HepG2 from the Shiitake Medicinal Mushroom, Lentinus edodes C91–3 (Agaricomycetes), Transcriptome JF International Journal of Medicinal Mushrooms JO IJM YR 2018 FD 2018-11-30 VO 20 IS 12 SP 1163 OP 1172 K1 Lentinus edodes K1 transcriptome K1 Latcripin-4 RCC1 and ANK domain protein K1 antitumor K1 medicinal mushrooms AB The recombinant protein of Latcripin-4 regulator of chromosome condensation 1 (RCC1) and ankyrin (ANK) domains were expressed and the antitumor activity of Latcripin-4 on HepG2 cells was studied. First, the Latcripin-4 transcript was selected from the medicinal mushroom Lentinus edodes C91–3 transcriptome by bioinformatics. Then the full-length gene of Latcripin-4 was isolated with 3'-full rapid amplification of cDNA ends (RACE) and 5'-full RACE methods according to the transcriptome. The RCC1 and ANK domains from the full-length gene were selected and inserted into the expression vector pET-32a (+) and expressed in Escherichia coli Rosetta-gami (DE3). Western blotting indicated that the protein was expressed successfully. The biological function of Latcripin-4 RCC1 and ANK domain protein on HepG2 cells was studied with the CCK-8 assay. All results demonstrated that Latcripin-4 RCC1 and ANK domain protein can inhibit the growth of human HepG2 liver cancer cells, which brings new insights to identifying antitumor proteins from medicinal food for cancer therapy. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2c6ab3993645ca9e,5b1694f16d976987.html