RT Journal Article ID 3b73a6430bf83bed A1 Kashina, Svetlana A1 Villavicencio, Lerida Liss Flores A1 Zaina, Silvio A1 Ordaz, Marco Balleza A1 Sabanero, Gloria Barbosa A1 Fujiyoshi, Victor Tsutsumi A1 Lopez, Myrna Sabanero T1 Activity of Extracts from Submerged Cultured Mycelium of Winter Mushroom, Flammulina velutipes (Agaricomycetes), on the Immune System In Vitro JF International Journal of Medicinal Mushrooms JO IJM YR 2016 FD 2016-03-09 VO 18 IS 1 SP 49 OP 57 K1 medicinal mushrooms K1 Flammulina velutipes K1 monocytes K1 macrophages K1 glycans AB Extracts from submerged cultured mycelium of two strains of Flammulina velutipes, a popular culinary mushroom, were obtained by ultrasound and tested in vitro to determine their activity in innate immunity (monocytes/ macrophages). In addition, polyclonal antibodies against the extracts were produced. Both extracts have similar glycoproteins that contain mannose and glucose but have different glycoproteins with galactoseamine units. Two novel immunogenic glycoproteins with molecular weights of 32 and 25 kDa have been revealed. It is thought that these proteins are produced only by submerged cultured mycelium. Both extracts show immune-enhancing activity based on the significant modification of various parameters such as cytokine production, phagocytosis, and reactive oxygen species production. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,33df00905b660ea5,3b73a6430bf83bed.html