RT Journal Article
ID 12a046c5473de19a
A1 Lo, Yu-Chang 
A1 Chien, Shih-Chang 
A1 Mishchuk, Darya O. 
A1 Slupsky, Carolyn M. 
A1 Mau, Jeng-Leun
T1 Quantification of Water-Soluble Metabolites in Medicinal Mushrooms Using Proton NMR Spectroscopy
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2016
FD 2016-08-30
VO 18
IS 5
SP 413
OP 424
K1 medicinal mushrooms
K1 NMR
K1 principal component analysis
K1 water-soluble metabolite
AB The water-soluble metabolites in 5 mushrooms were identified and quantified using proton nuclear
magnetic resonance (NMR) spectroscopy and software for targeted metabolite detection and quantification. In total,
35 compounds were found in <i>Agaricus brasiliensis</i>, 25 in <i>Taiwanofungus camphoratus</i>, 23 in <i>Ganoderma lucidum</i> (Taiwan) and <i>Lentinus edodes</i>, and 16 in <i>G. lucidum</i> (China). Total amounts of all identified metabolites in <i>A. brasiliensis, T. camphoratus, G. lucidum, G. lucidum</i> (China), and <i>L. edodes</i> were 149,950.51, 12,834.18, 9,549.09, 2,788.41, and 111,726.51 mg/kg dry weight, respectively. These metabolites were categorized into 4 groups: free amino acids and derivatives, carbohydrates, carboxylic acids, and nucleosides. Carbohydrates were the most abundant metabolites among all 4 groups, with mannitol having the highest concentration among all analyzed metabolites (848–94,104 mg/kg dry weight). Principal components analysis (PCA) showed obvious distinction among the metabolites of the 5 different kinds of mushrooms analyzed in this study. Thus PCA could provide an optional analytical way of identifying and recognizing the compositions of flavor products. Furthermore, the results of this study demonstrate that NMRbased metabolomics is a powerful tool for differentiating between various medicinal mushrooms.

PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,0cbef6a12a779453,12a046c5473de19a.html