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Visualization, Image Processing and Computation in Biomedicine

Publicou 4 edições por ano

ISSN On-line: 2162-3511

Elementary Morphometric Labs-on-a-Chip Based on Hemocytometric Chambers with Radiofrequency Culture Identification and Relay of Spectrozonal Histochemical Monitoring

Volume 2, Edição 1, 2013,
DOI: 10.1615/VisualizImageProcComputatBiomed.2013005968
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RESUMO

An accessible design of autonomous labs-on-the-chip, which do not require a special reader but use grid hemocytometric counting chambers for sectioning the detected cells, isolated by a special device for cell sedimentation, is proposed. A system for automated radiofrequency (RF) identification of chambers in long-term storage conditions, which provides new morphometric data at various stages of cultivation or biomonitoring, is proposed. A new diffraction method for calculating and fingerprinting cellular structures in varying environmental conditions is described. Experimental data on the pilot testing of Fuchs-Rosenthal, Buerker, Neubauer, Makler, and Thoma chambers for the described technique are presented. The applicability of these devices for coherent-densitometric indication either of concentration dynamics in cell culture or of suspension and morphogenetic processes in them for laminar conditions is shown. A self-learning program for cellular pattern recognition and precision cytophotometry based on a lab-on-a-chip with micrometric object calibration relative to the counting net is demonstrated. A hybrid in vitro/in silico method of morphogenesis monitoring in cell cultures is introduced.

CITADO POR
  1. Orekhov T. K., Gradov O. V., Digital Spectrozonal and Multispectral Lens-Less Devices with Spectrophotometric Temperature Calibration GUIs for Dairy Farming and Qualimetry of Diary Products, in Informatics and Cybernetics in Intelligent Systems, 228, 2021. Crossref

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