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International Journal on Algae
SJR: 0.219 SNIP: 0.261 CiteScore™: 0.24

ISSN Imprimir: 1521-9429
ISSN On-line: 1940-4328

International Journal on Algae

DOI: 10.1615/InterJAlgae.v18.i3.70
pages 279-286

The Medium for Intensive Culture of the Diatom Cylindrotheca closterium (Ehrenb.) Reimann et Lewin (Bacillariophyta)

V. I. Ryabushko
A.O. Kovalevsky Institute of Marine Biological Researches, 2 Nakhimov Pr., Sevastopol 99001, Crimea
S. N. Zheleznova
A.O. Kovalevsky Institute of Marine Biological Researches, 2 Nakhimov Pr., Sevastopol 99001, Crimea
R. G. Gevorgiz
A.O. Kovalevsky Institute of Marine Biology, 2, Nakhimov Ave., Sevastopol 99011, Crimea
N. I. Bobko
A.O. Kovalevsky Institute of Marine Biology, 2, Nakhimov Ave., Sevastopol 99011, Crimea
A.S. Lelekov
A.O. Kovalevsky Institute of Marine Biology, 2, Nakhimov Ave., Sevastopol 99011, Crimea

RESUMO

The proposed medium was developed specially for intensive culture of the diatom Cylindrotheca closterium. The averages of nitrogen, phosphorus, and silicon demand for the culture were calculated using data from the chemical analysis of the microalgal biomass. Growth limitation by nitrogen provoked agglutination of the microalgal cells. High concentrations of iron did not inhibit growth of C. closterium. Organic nitrogen present in the medium can be due to biosynthesis of exometabolites. During the stationary growth phase, the total and organic nitrogen in the medium were evaluated 28 mg·L-1 and 17.8 mg·L-1, correspondingly. The average loss of nitrogen in the culture was estimated at 10%. The maximal dry biomass harvested from the diatom was 4.6 g·L-1 and the productivity − 1 g·L-1·d-1. The new medium allows growing dense cultures of C. closterium with a larger biomass and therefore a proportionally larger yield of valuable biologically active substances, e.g., polyunsaturated fatty acids and carotenoids, primarily fucoxanthin.