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International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v8.i4.70
pages 361-376

Study of Hypsizygus marmoreus (Peck) Bigel. and Grifola frondosa (Dicks.: Fr.) S.F. Gray: Cultural-Morphological Peculiarities, Growth Characteristics, Qualitative Enzymatic Activity, and Resistance to Fungal Pest Contamination

Eden Akavia
International Center for Cryptogamic Plants and Fungi, Institute of Evolution University of Haifa, Mount Carmel, Haifa 31905, Israel
Alexander Beharav
International Center for Cryptogamic Plants and Fungi, Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel
Eviatar D. Nevo
Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, Institute of Evolution, University of Haifa, 199 Abba Khousi Ave., Mt. Carmel, Haifa 3498838, Israel

RESUMO

Hypsizygus marmoreus (HM) and Grifola frondosa (GF) are two highly praised culinary-medicinal mushrooms with good taste, texture, and fragrance. They possess health-enhancing properties and good commercial potential worldwide. We aimed to develop biotechnologically viable cultivation methods for these mushrooms, and to reveal the taxonomic significance of several growth and macro- and micromorphological characteristics of vegetative mycelia. Among the HM strains, we have documented vegetative mycelium with incrustations, networks of vegetative hyphae, some with short side protuberances, arthroconidia, some of them connected as chains, chlamydospores and intercalary chlamydospores, and clamp connections. GF structures included vegetative hyphae with clamps, anastomoses, and terminal chlamydospores. We have found that HM strains favored malt extract agar (MEA) and wort agar (WA) the most, with pH of 5.5 and 6.0. A growth coefficient of 47.3 was the highest and belonged to HM strain 571. Among GF strains, potato dextrose agar (PDA) was the most favored media at pH 4.5; GC of 58.2 was the highest and belonged to GF strain 924. In the solid-state measurement of laccase activity on guaiacol, we have found that enzyme secretion was associated with the agar media, or guaiacol, and not with the supplement, sawdust, or straw. Other enzymes demonstrated very low activity. When observing the relationship between the study mushroom mycelia and pest fungi, we have found that in all strains of the study mushrooms Aspergillus versicolor formed a border with GF and later overgrew it; HM formed a border with A. versicolor and later overgrew it; and Penicillium brevicompactum inhibited both HM and GF. Both mushrooms and pests did not form any contact with each other during these studies. The above-mentioned characteristics could be useful in culture for in vitro identification protocol of screened species during their biotechnological cultivation process.