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International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v8.i4.50
pages 343-350

Simultaneous Separation and Identification of Nucleosides in Cultured Cordyceps sinensis (Berk.) Sacc. (Ascomycetes) Mycelia by Using the HPLC-DAD-MS Technique

Xian-guo He
Mushroom Science Laboratory, 1025 Conger St. #6, Eugene, OR 97402 USA
Charles Johnson
Department of Chemistry, University of Oregon, Eugene, OR 97402, USA
John Seleen
Mushroom Science Laboratory, JHS Natural Products, P.O. Box 50398, Eugene, OR 97405, USA

RESUMO

Four nucleoside compounds (uridine, guanosine, adenosine, and 3’-deoxyadenosine) in the extract of cultured Cordyceps sinensis mycelia have been separated and unambiguously identified on line by using combined the high-performance liquid chromatography, diode array detection, and mass spectra (HPLC-DAD-MS) technique. The identification was based on the comparison of their HPLC Rt (retention time) values, UV (ultraviolet) spectra, and MS (mass spectra) with data of the authentic compounds. This method has provided a useful fingerprint for the comparison of different species of genus Cordyceps and their different cultivated mycelia.