Inscrição na biblioteca: Guest
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa
International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v19.i5.70
pages 457-465

A Validated Reverse-Phase HPLC Method for Quantitative Determination of Ganoderic Acids A and B in Cultivated Strains of Ganoderma spp. (Agaricomycetes) Indigenous to India

M. Ramakrishna
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India
D. Rajesh Babu
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India
S. S. Veena
Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore, Karnataka, India
Meera Pandey
Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore, Karnataka, India
Nageswara Rao
Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam Campus, Puttaparthi, Andhra Pradesh, India

RESUMO

Twenty isolates of Ganoderma spp. indigenous to India were used in this study. A reverse-phase high-performance liquid chromatography (HPLC) method was used to quantify 2 of the most bioactive triterpenes, ganoderic acid A (GAA) and ganoderic acid B (GAB), among the cultivated Ganoderma spp. The HPLC analysis was performed on an Agilent 1260 Infinity HPLC system with a Zorbax C18 column, using gradient elution of acetonitrile and 0.1% acetic acid. The detection wavelength was set at 254 nm, with a flow rate of 0.6 mL/min. The method was validated according to the guidelines of International Conference on Harmonisation and produced satisfactory results. The amount of GAA varied from 827.50 to 2010.36 µg/g, whereas GAB varied from 16.64 to 916.89 µg/g. The developed method is simple, specific, accurate, and precise, and can be useful for qualitative and quantitative evaluation of ganoderic acids.


Articles with similar content:

Isolation and Characterization of Bioactive Metabolites from Fruiting Bodies and Mycelial Culture of Ganoderma oerstedii (Higher Basidiomycetes) from Mexico
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 6
Jorge Suarez-Medellin, Cesar Espinoza, Manuel Norte, Angel Ramos-Ligonio, Jose J. Fernandez, Ángel Trigos, Guillermo Mendoza
Morphological Characteristics of Monokaryotic and Dikaryotic Collections of Three Medicinal Coprinellus Species (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 7
Susanna M. Badalyan
Cultural and Metabolomic Studies of a New Phtalides Producer, Lignomyces vetlinianus (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 11
Nadezhda V. Psurtseva, Katerina V. Sazanova, Alexey L. Shavarda
Simultaneous Separation and Identification of Nucleosides in Cultured Cordyceps sinensis (Berk.) Sacc. (Ascomycetes) Mycelia by Using the HPLC-DAD-MS Technique
International Journal of Medicinal Mushrooms, Vol.8, 2006, issue 4
John Seleen, Charles Johnson, Xian-guo He
Complex Enzyme-Assisted Extraction, Purification, and Antioxidant Activity of Polysaccharides from the Button Mushroom, Agaricus bisporus (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 10
Xiulian Yin, Qinghong You, Xinghai Zhou