Publicou 12 edições por ano
ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344
Indexed in
Extraction Optimization, Composition Analysis, and Antioxidation Evaluation of Polysaccharides from White Jelly Mushroom, Tremella fuciformis (Tremellomycetes)
RESUMO
We investigated the sonication-assisted extraction (SAE) conditions of Tremella fuciformis polysaccharides. Single-factor experiments showed that the optimal sonication intensity was 6 W/cm2; the other SAE conditions were further optimized using response surface experimental design. A Box-Behnken experiment exhibited that the optimal SAE conditions were temperature, 85°C; ratio of liquid to solid, 46 mL/g; and sonication time, 2 hours. The maximal polysaccharide yield (8.95 g/100 g) was obtained under the optimal extraction conditions. Polysaccharides extracted by sonication or traditional hot water extraction (TWE) were further separated and purified using DEAE-Sepharose and Sephadex G-100 column chromatography. Polysaccharide fractions (SAE-1, SAE-2, TWE-1, and TWE-2) were obtained; their molecular weights were 9.85 × 104, 6.64 × 104, 4.75 × 105, and 2.81 × 105 Da, respectively. The total sugar content of SAE-1 and SAE-2 was lower and the protein and nucleic acid contents were higher than those of TWE-1 and TWE-2. In addition, SAE-1 and SAE-2 showed stronger Fe2+-chelating activities and superoxide radical scavenging activities than TWE-1 and TWE-2. This suggests that polysaccharides extracted by sonication possess high antioxidant activities.
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Wu Yu-ji, Wei Zheng-xun, Zhang Fu-ming, Linhardt Robert J., Sun Pei-long, Zhang An-qiang, Structure, bioactivities and applications of the polysaccharides from Tremella fuciformis mushroom: A review, International Journal of Biological Macromolecules, 121, 2019. Crossref
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Yang Dandan, Liu Yong, Zhang Lijuan, Tremella polysaccharide: The molecular mechanisms of its drug action, in Glycans and Glycosaminoglycans as Clinical Biomarkers and Therapeutics - Part B, 163, 2019. Crossref
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Liu Dongmei, Pujiana Dwi, Wang Yuanyuan, Zhang Zhaosong, Zheng Liesheng, Chen Liguo, Ma Aimin, Comparative transcriptomic analysis identified differentially expressed genes and pathways involved in the interaction between Tremella fuciformis and Annulohypoxylon stygium, Antonie van Leeuwenhoek, 112, 11, 2019. Crossref
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Qian Lei, Liu Han, Li Tao, Liu Yihan, Zhang Zhijun, Zhang Yeni, Purification, characterization and in vitro antioxidant activity of a polysaccharide AAP–3–1 from Auricularia auricula, International Journal of Biological Macromolecules, 162, 2020. Crossref
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Yu Caiyuan, Fang Yan, Huang Weiwei, Lei Peng, Xu Xiaoqi, Sun Dafeng, Wu Lingtian, Xu Hong, Li Sha, Effect of surfactants on the production and biofunction of Tremella fuciformis polysaccharide through submerged fermentation, LWT, 163, 2022. Crossref
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Gürgen Ayşenur, Sevindik Mustafa, Application of artificial neural network coupling multiobjective particle swarm optimization algorithm to optimize Pleurotus ostreatus extraction parameters , Journal of Food Processing and Preservation, 2022. Crossref
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Huang Qian, Liu Yu, Deng Yongfei, Yang Bin, Guo Ruixue, Jin Xiaobao, Zhou Lin, Preparation and Antioxidant Activity In Vitro of Fermented Tremella fuciformis Extracellular Polysaccharides, Fermentation, 8, 11, 2022. Crossref