Publicou 12 edições por ano
ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344
Indexed in
Effect of Selenium-Enriched Agaricus bisporus (Higher Basidiomycetes) Extracts, Obtained by Pressurized Water Extraction, on the Expression of Cholesterol Homeostasis Related Genes by Low-Density Array
RESUMO
Culinary-medicinal mushrooms are able to lower blood cholesterol levels in animal models by different mechanisms. They might impair the endogenous cholesterol synthesis and exogenous cholesterol absorption during digestion. Mushroom extracts, obtained using pressurized water extractions (PWE) from Agaricus bisporus basidiomes, supplemented or not supplemented with selenium, were applied to HepG2 cell cultures to study the expression of 19 genes related to cholesterol homeostasis by low-density arrays (LDA). Only the PWE fractions obtained at 25°C showed 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) inhibitory activity. Besides the enzymatic inhibition, PWE extracts may downregulate some of the key genes involved in the cholesterol homeostasis, such as the squalene synthase gene (FDFT1), since its mRNA expression falls by one third of its initial value. In summary, A. bisporus extracts may also modulate biological cholesterol levels by molecular mechanisms further than the enzymatic way previously reported.
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Mleczek Mirosław, Siwulski Marek, Rzymski Piotr, Budzyńska Sylwia, Gąsecka Monika, Kalač Pavel, Niedzielski Przemysław, Cultivation of mushrooms for production of food biofortified with lithium, European Food Research and Technology, 243, 6, 2017. Crossref
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Gil-Ramírez Alicia, Morales Diego, Soler-Rivas Cristina, Molecular actions of hypocholesterolaemic compounds from edible mushrooms, Food & Function, 9, 1, 2018. Crossref
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Zhao Meng, Luo Ting, Zhao Zixuan, Rong Han, Zhao Guohua, Lei Lin, Food Chemistry of Selenium and Controversial Roles of Selenium in Affecting Blood Cholesterol Concentrations, Journal of Agricultural and Food Chemistry, 69, 17, 2021. Crossref