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International Journal of Medicinal Mushrooms
Fator do impacto: 1.423 FI de cinco anos: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344

Volumes:
Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v2.i4.110
9 pages

Ganoderma lucidum (Curt.: Fr.) P. Karst. (Aphyllophoromycetideae) Inhibits Proliferation of Human Peripheral Blood Lymphocytes by Blocking lnterleukin-2 Secretion

Ha Won Kim
Department of Life Sciences, University of Seoul Dongdaemun-gu, Jennong-dong 90, Seoul 130-743, South Korea
Mi Ja Shim
College of Pharmacy, Seoul National University, Seoul 151-742; and Department of Life Science, University of Seoul, Seoul 130-743, Korea
Byong Kak Kim
College of Pharmacy Seoul National University Seoul 151-724; and Department of Life Science, University of Seoul, Seoul 130-743, South Korea

RESUMO

Ganoderma lucidum is a famous medicinal mushroom that has various immunomodulating activities such as inhibition of antibody production, immunosuppressive antiviral activity, and inhibition of histamine release. However, there are few reports on the direct immunomodulating effects of this mushroom on human peripheral blood immunocompetent cells. For elucidation of the immunosuppressive mechanism of this mushroom, a methanolic (GLA) extract of its basidiocarps was fractionated into six parts. When each of the six fractions was applied to the adherent and nonadherent cells of human peripheral blood mononuclear cells (PBMCs), proliferative arrest was observed and growth arrest was stronger in the nonadherent cells than in the adherent cells. As the chloroform fraction of the fungus (GLE) arrested most strongly, it was further purified by silica gel column chromatography into seven subfractions. Among them, GLE1 fraction, the highest Rf value fraction, inhibited cell proliferation in a concentration-dependent manner. Antiproliferative activity of the GLE1 fraction was also observed in human allogeneic mixed lymphocyte culture (MLC). When secreted interleukin-2 (IL-2) of the culture supernatant of the MLC was measured by enzyme-linked immunosorbent assay (ELISA), the GLE1 fraction was found to inhibit IL-2 secretion, which was comparable to the effect of the immunosuppresive agent cyclosporin A. These data indicate that antiproliferating activity of the GLE1 fraction blocked IL-2 secretion. The GLE1 fraction of G. lucidum therefore may have the potential to be developed into an immunomodulating agent.


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