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Critical Reviews™ in Eukaryotic Gene Expression

Publicou 6 edições por ano

ISSN Imprimir: 1045-4403

ISSN On-line: 2162-6502

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.6 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 2.2 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00058 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.33 SJR: 0.345 SNIP: 0.46 CiteScore™:: 2.5 H-Index: 67

Indexed in

Recombinant Laccase: II. Medical Biosensor

Volume 22, Edição 3, 2012, pp. 197-203
DOI: 10.1615/CritRevEukarGeneExpr.v22.i3.30
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RESUMO

Langmuir-Blodgett (LB) technology was used to build a high-sensitivity enzyme-based biosensor for medical purposes. Recombinant fungal laccase from Rigidoporous lignosus, as previously described, was used to catalyze a widely used antidepressant in a micromolar range, namely, clomipramine. The topological properties of the laccase thin film were characterized via LB π-A isotherm and AFM (mean roughness 8.22 nm, compressibility coefficient 37.5 m/N). The sensitivity of the biosensor was investigated via UV spectroscopy, and linearity was found in the absorbance peak shift at 400 nm at drug concentration varying up to 20 uM. The enzyme kinetics was subsequently investigated with potentiometric and amperometric measurements, and we found electronic transfer of at least 1 electron, ks 0.57 s−1, diffusion coefficient 3 × 10−6 cm2/s, Kcat t 6825.92 min−1, KM 4.1 uM, Kcat /KM 2.8 × 107 mol−1 s−1, sensitivity of 440 nA/uM, maximum velocity 1706.48 nA/s, and response time less than 5 s. The amperometric and potentiometric measurements were repeated after a month, confirming the stability of the biosensor.

CITADO POR
  1. Bragazzi Nicola, Rethinking psychiatry with OMICS science in the age of personalized P5 medicine: ready for psychiatome?, Philosophy, Ethics, and Humanities in Medicine, 8, 1, 2013. Crossref

  2. Nicolini Claudio, Bruzzese Debora, Cambria Maria Teresa, Bragazzi Nicola Luigi, Pechkova Eugenia, Recombinant Laccase: I. Enzyme cloning and characterization, Journal of Cellular Biochemistry, 114, 3, 2013. Crossref

  3. Pechkova Eugenia, Bragazzi Nicola Luigi, Nicolini Claudio, Advances in Nanocrystallography as a Proteomic Tool, in Proteomics in Biomedicine and Pharmacology, 95, 2014. Crossref

  4. Ahamad Nur Uddin, Al-Amin Md., Ianoul Anatoli, A Simple Approach to Engineer SERS Substrates and Plasmonic Thin Film, Journal of Nanoparticles, 2014, 2014. Crossref

  5. Nicolini Claudio, Adami Manuela, Sartore Marco, Bragazzi Nicola, Bavastrello Valter, Spera Rosanna, Pechkova Eugenia, Prototypes of Newly Conceived Inorganic and Biological Sensors for Health and Environmental Applications, Sensors, 12, 12, 2012. Crossref

  6. Zhu Changwei, Bao Guangwen, Huang Shun, Optimization of laccase production in the white-rot fungusPleurotus ostreatus(ACCC 52857) induced through yeast extract and copper, Biotechnology & Biotechnological Equipment, 30, 2, 2016. Crossref

  7. Ielasi Francesco S., Hirtz Michael, Sekula-Neuner Sylwia, Laue Thomas, Fuchs Harald, Willaert Ronnie G., Dip-Pen Nanolithography-Assisted Protein Crystallization, Journal of the American Chemical Society, 137, 1, 2015. Crossref

  8. Spera Rosanna, Festa Fernanda, Bragazzi Nicola L., Pechkova Eugenia, LaBaer Joshua, Nicolini Claudio, Conductometric Monitoring of Protein–Protein Interactions, Journal of Proteome Research, 12, 12, 2013. Crossref

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