Inscrição na biblioteca: Guest
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa
Critical Reviews™ in Eukaryotic Gene Expression
Fator do impacto: 2.156 FI de cinco anos: 2.255 SJR: 0.649 SNIP: 0.599 CiteScore™: 3

ISSN Imprimir: 1045-4403
ISSN On-line: 2162-6502

Volume 30, 2020 Volume 29, 2019 Volume 28, 2018 Volume 27, 2017 Volume 26, 2016 Volume 25, 2015 Volume 24, 2014 Volume 23, 2013 Volume 22, 2012 Volume 21, 2011 Volume 20, 2010 Volume 19, 2009 Volume 18, 2008 Volume 17, 2007 Volume 16, 2006 Volume 15, 2005 Volume 14, 2004 Volume 13, 2003 Volume 12, 2002 Volume 11, 2001 Volume 10, 2000 Volume 9, 1999 Volume 8, 1998 Volume 7, 1997 Volume 6, 1996 Volume 5, 1995 Volume 4, 1994

Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukaryotGeneExpr.v13.i24.60
16 pages

The Effects of Colony-Stimulating Factor-1 (CSF-1) on the Development of Osteoclasts and Their Expression of Tartrate-Resistant Acid Phosphatase (TRAP) in Toothless (tl-osteopetrotic) Rats

Maria Norgard
Division of Pathology, Department of Laboratory Medicine, Karolinska Institutet, Huddinge University Hospital, S-141 86 Huddinge, Sweden
Sandy C. Marks, Jr.
Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655, USA
Finn P. Reinholt
Institute of Pathology, Rikshospitalet University Hospital, University of Oslo, Oslo, Norway
Goran Andersson
Division of Pathology, Department of Laboratory Medicine, Karolinska Institutet, Huddinge University Hospital, S-141 86 Huddinge, Sweden


The osteopetrotic mutation toothless (tl) in the rat is characterized by a limited number of osteoclasts with reduced amounts and/or activity of tartrate-resistant acid phosphatase (TRAP). Treatment of tl/tl mutants with the cytokine colony-stimulating factor (CSF)-1 increases both osteoclast number and enzyme activity, consistent with a loss-of-function mutation in the CSF-1 gene recently detected in this mutant. We have pursued these observations to demonstrate that there is a dose-dependent increase in osteoclast number, but not to normal levels. Osteoclasts in CSF-1–treated tl/tl mutants are large, have well-developed clear zones and ruffled borders, and secrete TRAP into resorption lacunae. The expression of TRAP mRNA, protein, and enzyme activity per bone appear normal after CSF-1 treatment. However, in contrast to the predominantly apical intracellular distribution in normal osteoclasts, an enrichment of TRAP enzyme activity in osteoclasts of CSF-1–treated tl/tl mutants is observed in the basal part of the cell. Our observations suggest that the CSF-1–treated mutant bones contain an abundance of mature osteoclasts, actively expressing markers for osteoclasts such as TRAP, cathepsin K, and matrix metalloproteinase (MMP)-9. Accumulation of TRAP at the end of the endocytic pathway in mature osteoclasts formed during CSF-1 treatment suggests that the TRAP enzyme has a rapid turnover in these highly active cells and uses a transcytotic pathway.

Articles with similar content:

Molecular Mechanisms of Tumor-Bone Interactions in Osteolytic Metastases
Critical Reviews™ in Eukaryotic Gene Expression, Vol.10, 2000, issue 2
Theresa A. Guise, John M. Chirgwin
Chemoprevention of Colon Cancer through Inhibition of Angiogenesis and Induction of Apoptosis by Nonsteroidal Anti-Inflammatory Drugs
Journal of Environmental Pathology, Toxicology and Oncology, Vol.35, 2016, issue 3
Sankar Nath Sanyal, Preety Ghanghas, Chandan Rana, Shelly Jain
Induction of Apoptosis and Chemosensitization by the Histone Deacetylase Inhibitor Trichostatin in Hepatocellular Carcinoma Cells: Molecular Analysis and RKIP Levels
Forum on Immunopathological Diseases and Therapeutics, Vol.2, 2011, issue 2
Luigi Inguglia, Manuela Labbozzetta, Paola Poma, Monica Notarbartolo, Natale D'Alessandro
Growth Hormone Receptor and Insulin-like Growth Factor-I Immunoreactivity in Osteoclast-like Cells During Tooth Eruption in the Toothless (Osteopetrotic) Rat Following Treatment with Colony-Stimulating Factor-1
Critical Reviews™ in Eukaryotic Gene Expression, Vol.13, 2003, issue 2-4
Arosha Weerakoon, Sandy C. Marks, Jr., Anne L. Symons
Modification of Thymocytes Membrane Radiooxidative Damage and Apoptosis by Eugenol
Journal of Environmental Pathology, Toxicology and Oncology, Vol.23, 2004, issue 2
Kaushala Prasad Mishra, Badri Narain Pandey