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Critical Reviews™ in Eukaryotic Gene Expression
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Critical Reviews™ in Eukaryotic Gene Expression

DOI: 10.1615/CritRevEukaryotGeneExpr.2019029029
pages 305-317

Bacterial Expression and Characterization of Recombinant β-Xylosidase from the Thermophilic Xylanolytic Bacterium Bacillus sp

Ghazaleh Gharib
Nanotechnology, Research and Application Center, Sabanci University, Nanotechnology Research and Applied Center, SUNUM, Istanbul, Turkey
Amina Arif
Faculty of Life Sciences, University of Central Punjab, Lahore, Pakistan
Asma Zaidi
Institute of Biochemistry and Biotechnology, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan
Mahjabeen Saleem
Institute of Biochemistry and Biotechnology, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan

RESUMO

With the passage of time, energy sources are decreasing day by day. In order to meet the world's demand, much attention is being paid to the study of enzymes with xylanolytic activity as a potential means of generating energy. A thermophilic xylanolytic bacterium, Bacillus sp., was isolated from naturally decaying material by enrichment culture and serial dilution methods. The bacterium was grown in MH medium at 50°C and pH 7 for 10 h. The xylanolytic Bacillus sp. produced clear yellow haloes around the colonies in the presence of p-nitrophenyl beta-D-xylopyranoside (pNPX) as a substrate. After condition optimization, it was found that the organism produced the higher level of xylosidase activity after 14 h in the presence of arabinose as a carbon source and ammonium sulfate as a nitrogen source in the pH 7 medium of at 55°C. The maximum β-xylosidase activity after optimizing the culture condition was 5.0 U/mL. Later this thermophilic Bacillus sp. was used as a donor in cloning of the β-xylosidase gene. A genomic library of Bacillus sp. was prepared by digesting the genomic DNA of the Bacillus with the restriction endonuclease BamHI, ligating the fragments in the pUC18 cloning vector and then transforming the competent E. coli DH5α cells with the resultant chimeric plasmid. The β-xylosidase gene was identified by screening the transformants in duplicates on LB agar plates overlaid with pNPX as a substrate. Commercial production of β-xylosidase to be used as a methanol-producing enzyme can help to overcome fuel shortages.

Referências

  1. Christov LP, Myburgh J, O'Neill FH, Van Tonder A, Prior BA. Modification of the carbohydrate composition of sulfite pulp by purified and characterized P-xylanase and P-xylosidase ofAureobasidium pullulans. Biotechnol Prog. 1999;15(2):196-200.

  2. Shi H, Li X, Gu H, Zhang Y, Huang Y, Wang L, Wang F. Biochemical properties of a novel thermostable and highly xylose-tolerant P-xylosidase/a-arabinosidase from Thermotoga thermarum. Biotechnol Biofuels. 2013 Dec;6(1):27.

  3. Choengpanya K, Arthornthurasuk S, Wattana-amorn P, Huang WT, Plengmuankhae W, Li YK, Kongsaeree PT. Cloning, expression and characterization of P-xylosidase from Aspergillus niger ASKU28. Protein Expression Purification. 2015;115:132-40.

  4. Jordan DB, Stoller JR, Lee CC, Chan VJ, Wagschal K. Biochemical characterization of a GH43 P-xylosidase from Bacteroides ovatus. Appl Biochem Biotechnol. 2017;182(1):250-60.

  5. Matsuzawa T, Kaneko S, Kishine N, Fujimoto Z, Yaoi K. Crystal structure of metagenomic P-xylosidase/a-L-arabinofuranosidase activated by calcium. J Biochem. 2017 Feb 16;162(3):173-81.

  6. Tanaka T, Hirata Y, Nakano M, Kawabata H, Kondo A. Creation of cellobiose and xylooligosaccharides-coutilizing Escherichia coli displaying both P-glucosidase and P-xylosidase on its cell surface. ACS Synthetic Biol. 2013 Nov 8;3(7):446-53.

  7. Sengupta A, Zabala A, Tan SY, Broadstock A, Suryanarayanan TS, Gopalan V. Characterization of an ionic liquid-tolerant P-xylosidase from a marine-derived fungal endophyte. Biochem Cell Biol. 2017 May 19;95(5):585-91.

  8. Zhou J, Bao L, Chang L, Liu Z, You C, Lu H. Beta- xylosidase activity of a GH3 glucosidase/xylosidase from yak rumen metagenome promotes the enzymatic degradation of hemicellulosic xylans. Lett Appl Microbiol. 2012 Feb;54(2):79-87.

  9. Smaali I, Remond C, Skhiri Y, O'Donohue MJ. Biocatalytic conversion of wheat bran hydrolysate using an immobilized GH43 P-xylosidase. Bioresource Technol. 2009 Jan 1;100(1):338-44.

  10. Aftab MN, Zafar A, Awan AR. Expression of thermostable P-xylosidase in Escherichia coli for use in saccharification of plant biomass. Bioengineered. 2017 Sep 3;8(5):665-9.

  11. Zimbardi AL, Sehn C, Meleiro LP, Souza FH, Masui DC, Nozawa MS, Guimaraes LH, Jorge JA, Furriel RP. Optimization of P-glucosidase, P-xylosidase and xylanase production by Colletotrichum graminicola under solid-state fermentation and application in raw sugarcane trash saccharification. Int J Mol Sci. 2013;14(2):2875-902.

  12. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72(1-2):248-54.

  13. Beji A, Izard D, Gavini F, Leclerc H, Leseine-Delstanche M, Krembel J. A rapid chemical procedure for isolation and purification of chromosomal DNA from Gramnegative bacilli. Anal Biochem. 1987Apr 1;162(1):18-23.

  14. Feliciello I, Chinali G. A modified alkaline lysis method for the preparation of highly purified plasmid DNA from Escherichia coli. Anal Biochem. 1993 Aug 1;212(2):394-401.

  15. Maruthamuthu M, Jimenez DJ, van Elsas JD. Characterization of a furan aldehyde-tolerant P-xylosidase/a-arabinosidase obtained through a synthetic metagenomics approach. J Appl Microbiol. 2017 Jul;123(1):145-58.

  16. Chen CC, Westpheling J. Partial characterization of the Streptomyces lividans xlnB promoter and its use for expression of a thermostable xylanase from Thermotoga maritima. Appl Environ Microbiol. 1998 Nov 1;64(11):4217-25.

  17. Michelin M, Maria de Lourdes TM, Ruzene DS, Silva DP, Vicente AA, Jorge JA, Terenzi HF, Teixeira JA. Xylanase and P-xylosidase production by Aspergillus ochraceus: new perspectives for the application of wheat straw autohydrolysis liquor. Appl Biochem Biotechnol. 2012 Jan 1;166(2):336-47.

  18. Banka AL, Guralp SA, Gulari E. Secretory expression and characterization of two hemicellulases, xylanase, and P-xylosidase, isolated from Bacillus subtilis M015. Appl Biochem Biotechnol. 2014 Dec 1;174(8):2702-10.

  19. Quintero D, Velasco Z, Hurtado-Gomez E, Neira JL, Contreras LM. Isolation and characterization of a thermostable P-xylosidase in the thermophilic bacterium Geobacillus pallidus. Biochim Biophys Acta: Proteins Characterization of Recombinant P-Xylosidase Proteomics. 2007 Apr 1;1774(4):510-8.

  20. Qian Y, Yomano LP, Preston JF, Aldrich HC, Ingram LO. Cloning, characterization, and functional expression of the Klebsiella oxytoca xylodextrin utilization operon (xynTB) in Escherichia coli. Appl Environ Microbiol. 2003 Oct 1;69(10):5957-67.

  21. La Grange DC, Pretorius IS, Claeyssens M, Van Zyl WH. Degradation of xylan to d-xylose by recombinant Saccharomyces cerevisiae coexpressing theaspergillus niger P-xylosidase (xlnD) and the Trichoderma reesei xylanase II (xyn2) genes. Appl Environ Microbiol. 2001;67(12):5512-9.

  22. Nieto-Dominguez M, de Eugenio LI, Barriuso J, Prieto A, de Toro BF, Canales-Mayordomo A, Martinez MJ. Novel pH-stable glycoside hydrolase family 3 P-xylosidase from Talaromyces amestolkiae: an enzyme displaying regioselective transxylosylation. Appl Environ Microbiol. 2015;81(18):6380-92.

  23. Correa JM, Christi D, Torre CL, Henn C, Conceifao- Silva JL, Kadowaki MK, Simao RD. High levels of P-xylosidase in Thermomyces lanuginosus: potential use for saccharification. Brazil J Microbiol. 2016 Sep;47(3):680-90.


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