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Plasma Medicine

Publicou 4 edições por ano

ISSN Imprimir: 1947-5764

ISSN On-line: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Action of an Argon/Water Vapor Plasma Jet in the Sterilization of Silicone Contaminated with Candida albicans

Volume 7, Edição 3, 2017, pp. 299-311
DOI: 10.1615/PlasmaMed.2017019495
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RESUMO

This work evaluates the influence of argon/water vapor plasma jet exposure time on surface sterilization of a silicone substrate contaminated with Candida albicans. For this purpose, gliding arc-type geometry was used to generate a nonthermal plasma jet at an applied power on the order of 20 W under water vapor carried by an argon flow of 5 L/min. C. albicans biofilms were grown on silicone plates with a 2cm2 area. Samples were treated with a plasmajet for different exposure times, resulting in the following sample groups: nontreated (A) or treated with plasma for 2 min (B), 5 min (C), 10 min (D), and 20 min (E). Experiments were performed in triplicate. To evaluate the action of the plasma jet on the contaminated samples, colony-forming units (CFUs) were counted before and after plasma exposure. Scanning electron microscopy (SEM) was used to analyze the morphological modification of the treated substrate, whereas Fourier transform infrared spectroscopy (FT-IR) was used to analyze the bare substrate after exposure to plasma. Optical emission spectroscopy was used to investigate the plasma species in the ultraviolet range. Results indicated reductions in CFUs of 42.4%, 74.2%, 83.7%, and 97.5% for groups B, C, D, and E, respectively. SEM images showed that groups D and E suffered more external cellular changes with shriveled, swollen, and/or deformed aspects. These effects can be attributed to reactive species in the plasma such as hydroxyl radicals (OH), which were increased considerably with the presence of water vapor in the mixture with argon gas. A quality change in the surface of the silicone material after 20 min of plasma treatment was observed with the results of the SEM and FT-IR analyses. Finally, the argon/water plasma jet promoted only a slight increase in substrate temperature (up to 310 K) for the investigated conditions, which is very convenient for thermal-sensitive material sterilization.

CITADO POR
  1. Doria A C O C, Figueira F R, de Lima J S B, Figueira J A N, Castro A H R, Sismanoglu B N, Petraconi G, Maciel H S, Khouri S, Pessoa R S, Inactivation of Candida albicans biofilms by atmospheric gliding arc plasma jet: effect of gas chemistry/flow and plasma pulsing, Plasma Research Express, 1, 1, 2018. Crossref

  2. de Oliveira Simone Maria Menegatti, da Silva Newton Soares, Sene Ana, Gandra Rinaldo Ferreira, Junges Daniele Schaab Boff, Ramos Marco Antonio Ramirez, Vieira Lucia, Comparative Study of Candida albicans Inactivation by Nonthermal Plasma on Stainless Steel with and without Diamond-like Carbon Film, ACS Omega, 4, 4, 2019. Crossref

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