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Critical Reviews™ in Biomedical Engineering

Publicou 6 edições por ano

ISSN Imprimir: 0278-940X

ISSN On-line: 1943-619X

SJR: 0.262 SNIP: 0.372 CiteScore™:: 2.2 H-Index: 56

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Super-Resolution Scanning Laser Microscopy Based on Virtually Structured Detection

Volume 43, Edição 4, 2015, pp. 297-322
DOI: 10.1615/CritRevBiomedEng.2016016445
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RESUMO

Light microscopy plays a key role in biological studies and medical diagnosis. The spatial resolution of conventional optical microscopes is limited to approximately half the wavelength of the illumination light as a result of the diffraction limit. Several approaches−including confocal microscopy, stimulated emission depletion microscopy, stochastic optical reconstruction microscopy, photoactivated localization microscopy, and structured illumination microscopy−have been established to achieve super-resolution imaging. However, none of these methods is suitable for the super-resolution ophthalmoscopy of retinal structures because of laser safety issues and inevitable eye movements. We recently experimentally validated virtually structured detection (VSD) as an alternative strategy to extend the diffraction limit. Without the complexity of structured illumination, VSD provides an easy, low-cost, and phase artifact−free strategy to achieve super-resolution in scanning laser microscopy. In this article we summarize the basic principles of the VSD method, review our demonstrated single-point and line-scan super-resolution systems, and discuss both technical challenges and the potential of VSD-based instrumentation for super-resolution ophthalmoscopy of the retina.

CITADO POR
  1. Lu Yiming, Wang Benquan, Pepperberg David R., Yao Xincheng, Stimulus-evoked outer segment changes occur before the hyperpolarization of retinal photoreceptors, Biomedical Optics Express, 8, 1, 2017. Crossref

  2. Gregor Ingo, Enderlein Jörg, Image scanning microscopy, Current Opinion in Chemical Biology, 51, 2019. Crossref

  3. Dong Hongzhou, Ao Mingwu, Yang Xianming, Liu Yong, Yang Chunping, Superresolution technology based on a heterodyne detection system, Applied Optics, 59, 10, 2020. Crossref

  4. Yao Xincheng, Lu Rongwen, Wang Benquan, Lu Yiming, Kim Tae-Hoon, Super-resolution ophthalmoscopy: Virtually structured detection for resolution improvement in retinal imaging, Experimental Biology and Medicine, 246, 3, 2021. Crossref

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