Publicou 6 edições por ano
ISSN Imprimir: 0278-940X
ISSN On-line: 1943-619X
Indexed in
Microphysiometry to Evaluate Real-Time Response of Mammary Epithelial Cells to IGF-I
RESUMO
The CytosensorТ Microphysiometer, a biosensor developed by Molecular Devices, was used to assay rapid binding activity of IGF-I for bovine mammary epithelial cell lines. Insulin-like growth factor-I (IGF-I) is a potent mitogen for mammary epithelial cells and has been implicated in breast cancer cell proliferation, a leading cause of cancer death of women in the U.S. today. IGF-I acts by binding to cell surface receptors. We are interested in how autocrine secretion might alter the activity and regulation of IGF-I.
Real-time changes in excretion of protons, which we assert results from IGF-I binding, are detected by the CytosensorТ Microphysiometer and can be correlated with cellular activity. We present IGF-I dose-dependent responses as well as correlated binding data to detect cell surface receptor concentration and thymidine incorporation results to determine cell proliferation following IGF-I stimulation. We examine the effect of insulin-like binding protein-3 (IGFBP-3) both in the presence and absence of IGF-I. We believe comparison of autocrine and paracrine environments for IGF-I stimulation, and the components contributing to the binding of IGF-I to the cell membrane receptor may provide pertinent information for the development of intervention schemes to slow or interrupt IGF-I binding to tumor cells and therefore cancer growth.
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GERMAN TANIA, BARASH ITAMAR, CHARACTERIZATION OF AN EPITHELIAL CELL LINE FROM BOVINE MAMMARY GLAND, In Vitro Cellular & Developmental Biology - Animal, 38, 5, 2002. Crossref
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Suchyta Steven P., Sipkovsky Sue, Halgren Robert G., Kruska Rachael, Elftman Michael, Weber-Nielsen Miriam, Vandehaar Michael J., Xiao Lan, Tempelman Robert J., Coussens Paul M., Bovine mammary gene expression profiling using a cDNA microarray enhanced for mammary-specific transcripts, Physiological Genomics, 16, 1, 2003. Crossref