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Critical Reviews™ in Biomedical Engineering
SJR: 0.207 SNIP: 0.376 CiteScore™: 0.79

ISSN Imprimir: 0278-940X
ISSN On-line: 1943-619X

Volumes:
Volume 47, 2019 Volume 46, 2018 Volume 45, 2017 Volume 44, 2016 Volume 43, 2015 Volume 42, 2014 Volume 41, 2013 Volume 40, 2012 Volume 39, 2011 Volume 38, 2010 Volume 37, 2009 Volume 36, 2008 Volume 35, 2007 Volume 34, 2006 Volume 33, 2005 Volume 32, 2004 Volume 31, 2003 Volume 30, 2002 Volume 29, 2001 Volume 28, 2000 Volume 27, 1999 Volume 26, 1998 Volume 25, 1997 Volume 24, 1996 Volume 23, 1995

Critical Reviews™ in Biomedical Engineering

DOI: 10.1615/CritRevBiomedEng.2018028254
pages 469-493

Scaffold-Free Spheroids Derived from Stem Cells for Tissue-Engineering Applications

Kaushik U. Desai
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, India
Pradnya M. Salve
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, India
Neha B. Sapkal
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, India
Jay R. Dave
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, India
Geetanjali B. Tomar
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, India

RESUMO

Tissue engineering has gained attention in the past decade due to its efficient interaction with the host system and potential therapeutic capabilities. Although scaffold-based approaches provide much needed mechanical strength and support to the regenerating tissue, they also invite foreign body reaction initiated by macrophages, causing inflammation and toxicity, and may also sometime interfere with the regeneration of indigenous tissue due to very slow degradation. Therefore, spheroids provide a promising tool for improving cell survival and for preserving cell-to-cell interaction. They have promptly gained popularity because of their ability to provide superior cellular heterogeneity, nutrient and oxygen gradients (replicating the original tissue), matrix deposition, and gene expression profiles. Because of their ability to differentiate into multiple cell lineages, stem cell–based spheroids have opened new avenues for future regenerative medicine. In this review we focus on various methods for fabrication of spheroids from stem cells and their application in regenerative approaches for different tissues/organs.