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Hydrobiological Journal
SJR: 0.227 SNIP: 0.575 CiteScore™: 0.24

ISSN Imprimir: 0018-8166
ISSN On-line: 1943-5991

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Hydrobiological Journal

DOI: 10.1615/HydrobJ.v48.i5.100
pages 107-112

Method of Intravital Morphometry of the Nucleated Erythrocytes of Fishes

A.Yu. Andreyeva
Institute of Biology of South Seas National Academy of Sciences of Ukraine Sevastopol, Ukraine
V. S. Mukhanov
A.O. Kovalevsky Institute of Biology of the Southern Seas, 2, Nakhimov Prosp., Sevastopol 99011, Crimea


The method of intravital morphology of fish erythrocytes using vital stains has been developed. For the nuclei staining fluorochrome SYBR Green 1 was used. The photos of erythrocytes in thick drop were made; pictures were analyzed by the ImageJ 1.44p software. Obtained values of length of the large and small axes of nuclei and cells were averaged and compared with values, measured on the blood smears stained according to the Pappenheim method. Differences did not exceed 2%.

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