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Hydrobiological Journal
SJR: 0.227 SNIP: 0.575 CiteScore™: 0.24

ISSN Imprimir: 0018-8166
ISSN On-line: 1943-5991

Volumes:
Volume 55, 2019 Volume 54, 2018 Volume 53, 2017 Volume 52, 2016 Volume 51, 2015 Volume 50, 2014 Volume 49, 2013 Volume 48, 2012 Volume 47, 2011 Volume 46, 2010 Volume 45, 2009 Volume 44, 2008 Volume 43, 2007 Volume 42, 2006 Volume 41, 2005 Volume 40, 2004 Volume 39, 2003 Volume 38, 2002 Volume 37, 2001 Volume 36, 2000 Volume 35, 1999 Volume 34, 1998

Hydrobiological Journal

DOI: 10.1615/HydrobJ.v35.i1.100
pages 81-84

The Efficiency of Estimating Total Number of Bacteria by Epifluorescence Microscopy

N. A. Potapova
Institute of Hydrobiology, National Academy of Sciences of Ukraine, Kiev, Ukraine

RESUMO

A new version of the epifluorescence technique for estimating the total numbers of bacteria was elaborated. The version was compared with direct counting of bacteria on nitrocellulose filters according to Razumov. The Razumov's method was shown to overestimate the total numbers of bacteria in water with a high concentration of detritus (suspended organic matter) and to underestimate this parameter when mineral components are prevalent in suspended matter. Estimates obtained with the two methods were positively associated in only natural waters with a relatively high functional activity of bacterioplankton.


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