Publicou 6 edições por ano
ISSN Imprimir: 1040-8401
ISSN On-line: 2162-6472
IF:
1.3
5-Year IF:
2.6
Eigenfactor:
0.00079
JCI:
0.24
SJR:
0.429
SNIP:
0.287
CiteScore™::
2.7
H-Index:
81
Indexed in
Measuring T Cell Responses by Flow Cytometry–Based Fluorescence In Situ Hybridization
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RESUMO
T cells produce a wide variety of effector molecules in response to infections, such as cytokines, chemokines, granzymes, and perforins. Because different stimuli promote the production of specific effector molecules, T cell responses come in different flavors. In addition, single-cell analysis of protein production revealed that T cells respond heterogeneously to activation. To unravel the regulatory mechanisms that determine T cell effector function, novel methods were developed that simultaneously measure protein levels with the corresponding mRNA. These flow cytometry-based fluorescence in situ hybridization (Flow-FISH) technologies allow for multiparameter analysis with single-cell resolution of both nucleic acids and proteins. Here, we review the currently available methods of Flow-FISH and describe the possible applications thereof, with a specific focus on T cells.
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Salerno Fiamma, Freen-van Heeren Julian J., Guislain Aurelie, Nicolet Benoit P., Wolkers Monika C., Costimulation through TLR2 Drives Polyfunctional CD8+ T Cell Responses, The Journal of Immunology, 202, 3, 2019. Crossref
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Freen-van Heeren Julian J., Nicolet Benoit P., Wolkers Monika C., Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells, in Immune Mediators in Cancer, 2108, 2020. Crossref
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Freen‐van Heeren Julian J., Addressing HIV ‐1 latency with Flow‐FISH : Finding, characterizing and targeting HIV ‐1 infected cells , Cytometry Part A, 99, 9, 2021. Crossref
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Freen‐van Heeren Julian J., Harnessing high‐throughput flow cytometric protein and RNA detection to enhance TIL therapy , Cytometry Part A, 99, 8, 2021. Crossref
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Freen‐van Heeren Julian J., Toll‐like receptor‐2/7‐mediated T cell activation: An innate potential to augment CD8 + T cell cytokine production , Scandinavian Journal of Immunology, 93, 5, 2021. Crossref
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Freen‐van Heeren Julian J., Post‐transcriptional control of T‐cell cytokine production: Implications for cancer therapy, Immunology, 164, 1, 2021. Crossref
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Freen-van Heeren Julian J., Flow-FISH as a Tool for Studying Bacteria, Fungi and Viruses, BioTech, 10, 4, 2021. Crossref
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Freen-van Heeren Julian J., Closing the Door with CRISPR: Genome Editing of CCR5 and CXCR4 as a Potential Curative Solution for HIV, BioTech, 11, 3, 2022. Crossref
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Freen‐van Heeren Julian Jamie, Investigating EBV biology with Flow‐FISH : Implications for EBV ‐mediated malignancies and the treatment thereof , Cytometry Part A, 2022. Crossref
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Osborne Douglas Grant, Domenico Joanne, Fujita Mayumi, Expression of IL-37 Induces a Regulatory T-Cell-like Phenotype and Function in Jurkat Cells, Cells, 11, 16, 2022. Crossref
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Freen‐van Heeren Julian J., FISHing for COVID ‐19: Studying SARS‐CoV ‐2 with single‐molecule FISH and flow‐FISH , Cytometry Part A, 2022. Crossref