Inscrição na biblioteca: Guest
Journal of Environmental Pathology, Toxicology and Oncology

Publicou 4 edições por ano

ISSN Imprimir: 0731-8898

ISSN On-line: 2162-6537

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 2.4 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 2.8 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.5 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00049 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.59 SJR: 0.429 SNIP: 0.507 CiteScore™:: 3.9 H-Index: 49

Indexed in

Culture of Human A375 Melanoma Cells in the Presence of Fibronectin Causes Expression of MMP-9 and Activation of MMP-2 in Culture Supernatants

Volume 27, Edição 2, 2008, pp. 135-145
DOI: 10.1615/JEnvironPatholToxicolOncol.v27.i2.60
Get accessGet access

RESUMO

Interactions between tumor cell surface integrin receptors and extracellular matrix (ECM) ligands play an important role in tumor development, affecting cell survival, proliferation, and migration. Integrin-ECM ligand interaction leads to phosphorylation of focal adhesion kinase (FAK) and activation of mitogen-activated protein kinase (MAPK) pathways. It has been reported that integrins also regulate expression and function of matrix metalloproteinases (MMPs). In this present work, we cultured human A375 melanoma cells in the presence of fibronectin to study fibronectin-integrin mediated modulation of MMP activity. Methods: A375 cells were cultured in serum-free culture medium (SFCM) in the presence of fibronectin (25 μg/0.75ml), SFCM was collected and gelatin zymography was performed. Western blot and RT-PCR were performed with A375 cells cultured in the presence of fibronectin. Results: Culture of A375 cells in the presence of fibronectin led to expression of MMP-9 and activation of MMP-2 within 2 h. When cells were treated with ERK inhibitor (PD98059) or PI-3K inhibitor (LY294002) and grown in the presence of fibronectin, MMP-9 expression and MMP-2 activation was inhibited. Tyrosine phosphorylation of FAK and ERK were increased in A375 cells grown in the presence of fibronectin. Increased MMP-9 mRNA expression and processing of MT1-MMP were also observed in A375 cells grown in the presence of fibronectin. Conclusions: Our findings indicate culture of A375 cells in SFCM in the presence of fibronectin perhaps generates a signaling cascade that leads to expression of MMP-9 and activation of MMP-2 in culture supernatants within 2 h. The signaling pathway activated is probably the FAK/ERK pathway.

CITADO POR
  1. Ganguly Kirat Kumar, Pal Sekhar, Moulik Shuvojit, Chatterjee Amitava, Integrins and metastasis, Cell Adhesion & Migration, 7, 3, 2013. Crossref

  2. Maity Gargi, Choudhury Paromita Roy, Sen Triparna, Ganguly Kirat Kumar, Sil Hrishikesh, Chatterjee Amitava, Culture of human breast cancer cell line (MDA-MB-231) on fibronectin-coated surface induces pro-matrix metalloproteinase-9 expression and activity, Tumor Biology, 32, 1, 2011. Crossref

  3. Maity Gargi, Fahreen Shabana, Banerji Aniruddha, Roy Choudhury Paromita, Sen Triparna, Dutta Anindita, Chatterjee Amitava, Fibronectin–integrin mediated signaling in human cervical cancer cells (SiHa), Molecular and Cellular Biochemistry, 336, 1-2, 2010. Crossref

  4. Ren Ye, Guo Fengjing, Chen Anmin, Deng Rui, Wang Jiang, Involvement of MMP-2 in adriamycin resistance dependent on ERK1/2 signal pathway in human osteosarcoma MG-63 cells, Journal of Huazhong University of Science and Technology [Medical Sciences], 32, 1, 2012. Crossref

  5. Shin Della S., Tokuda Emi Y., Leight Jennifer L., Miksch Connor E., Brown Tobin E., Anseth Kristi S., Synthesis of Microgel Sensors for Spatial and Temporal Monitoring of Protease Activity, ACS Biomaterials Science & Engineering, 4, 2, 2018. Crossref

  6. Gould Rothberg Bonnie E., Berger Aaron J., Molinaro Annette M., Subtil Antonio, Krauthammer Michael O., Camp Robert L., Bradley William R., Ariyan Stephan, Kluger Harriet M., Rimm David L., Melanoma Prognostic Model Using Tissue Microarrays and Genetic Algorithms, Journal of Clinical Oncology, 27, 34, 2009. Crossref

  7. Dawoud Marwa M., Jones Dylan T., Chelala Claude, Abdou Asmaa G., Dreger Sally A., Asaad Nancy, Abd El-Wahed Moshirah, Jones Louise, Expression Profile of Myoepithelial Cells in DCIS: Do They Change From Protective Angels to Wicked Witches?, Applied Immunohistochemistry & Molecular Morphology, 30, 6, 2022. Crossref

781 Visualizações do artigo 54 downloads de artigos Métricas
781 VISUALIZAÇÕES 54 TRANSFERÊNCIAS 7 Crossref CITAÇÕES Google
Scholar
CITAÇÕES

Artigos com conteúdo semelhante:

Culture of Human Fibrosarcoma HT-1080 Cells in Presence of Fibronectin Activates MMP-2 Journal of Environmental Pathology, Toxicology and Oncology, Vol.25, 2006, issue 4
Amitava Chatterjee, Jayati Chakrabarti, Aparna Mitra, Aniruddha Banerji
Cell Membrane-Associated MT1-MMP-Dependent Activation of Pro-MMP-2 in A375 Melanoma Cells Journal of Environmental Pathology, Toxicology and Oncology, Vol.24, 2005, issue 1
Amitava Chatterjee, Jayati Chakrabarti, Aparna Mitra, Aniruddha Banerji
Black Tea Polyphenol (Theaflavin) Downregulates MMP-2 in Human Melanoma Cell Line A375 by Involving Multiple Regulatory Molecules Journal of Environmental Pathology, Toxicology and Oncology, Vol.29, 2010, issue 1
Hrishikesh Sil, Triparna Sen, Amitava Chatterjee, Shuvojit Moulik
Cell Membrane-Associated MT1-MMP Dependent Activation of MMP-2 in SiHa (Human Cervical Cancer) Cells Journal of Environmental Pathology, Toxicology and Oncology, Vol.25, 2006, issue 4
Amitava Chatterjee, Jayati Chakrabarti, Aparna Mitra, Aniruddha Banerji
Effects of Allocryptopine on the Proliferation and Epithelial-Mesenchymal Transition of Oral Squamous Cell Carcinoma through m6A Mediated Hedgehog Signaling Pathway Journal of Environmental Pathology, Toxicology and Oncology, Vol.41, 2022, issue 2
Junxia Gong, Fang Zhang, Weidong Lan, Chunlin Wang
Portal Digital Begell Biblioteca digital da Begell eBooks Diários Referências e Anais Coleções de pesquisa Políticas de preços e assinaturas Begell House Contato Language English 中文 Русский Português German French Spain