Publicou 4 edições por ano
ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537
Indexed in
Generation of Reactive Oxygen Species and Endoplasmic Reticulum Stress by Dictyopteris undulata Extract Leads to Apoptosis in Human Melanoma Cells
RESUMO
In this study, we evaluated the hypothesis that a marine brown algae, Dictyopteris undulata ethanol extract (DUE), provokes apoptosis in a human melanoma cell line, A2058, via reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress. DUE inhibited A2058 cell proliferation and increased apoptotic body formation, as indicated by the presence of fragmented nuclei and the activation of caspase-3. Moreover, DUE-treated cells showed elevated ER staining, mitochondrial calcium cation (Ca2+) overloading, augmented levels of ER stress-related and cell death modulatory proteins, including RNA-dependent protein kinase-related ER kinase, phospho-inositol-requiring enzyme 1α, phospho-eukaryotic translation initiation factor 2α, and CCAAT/enhancer-binding protein-homologous protein, as well as increased intracellular ROS levels. However, the antioxidant N-acetyl cysteine reversed the elevated ROS levels, decreased apoptosis, and mitigated ER stress in A2058 cells following DUE treatment. These findings suggest that DUE treatment triggers apoptosis in human melanoma cells through a mechanism involving ER stress and ROS.
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Zatelli Gabriele Andressa, Philippus Ana Cláudia, Falkenberg Miriam, An overview of odoriferous marine seaweeds of the Dictyopteris genus: insights into their chemical diversity, biological potential and ecological roles, Revista Brasileira de Farmacognosia, 28, 2, 2018. Crossref
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Yu Chung-I, Chen Chung-Yi, Liu Wangta, Chang Po-Chih, Huang Chiung-Wei, Han Kuang-Fen, Lin In-Pin, Lin Mei-Ying, Lee Chien-Hsing, Sandensolide Induces Oxidative Stress-Mediated Apoptosis in Oral Cancer Cells and in Zebrafish Xenograft Model, Marine Drugs, 16, 10, 2018. Crossref
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Chandra Sonam, Qureshi Saba, Chopra Deepti, Dwivedi Ashish, Ray Ratan Singh, Involvement of Type‐I and Type‐II Photodynamic Reactions in Photosensitization of Fragrance Ingredient 2‐acetonaphthone, Photochemistry and Photobiology, 2022. Crossref