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ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537
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Naringin, a Natural Flavonoid, Modulates UVB Radiation-Induced DNA Damage and Photoaging by Modulating NER Repair and MMPS Expression in Mouse Embryonic Fibroblast Cells
RESUMO
We have proven that naringin, a phytonutrient, diminishes oxidative damage and inflammatory responses by modulating PPAR-γ expressions in ultraviolet-B radiation (UVB)-induced NIH-3T3 cells. However, the role of naringin against DNA damage, photoaging, and apoptosis in NIH-3T3 cells has yet to be studied, necessitating investigation. We show that Naringin pretreatment significantly reduces UVB-induced alkaline DNA damage and potentially modulates NER gene (XPC, TFIIH, XPE, ERCC1, and GAPDH) expression, thereby augmenting DNA repair. We determined experimentally that naringin pretreatment prevents UVB-induced nuclear fragmentation in NIH-3T3 cells, as well as altering UVB-induced apoptotic marker (Bax, BCl-2, Caspase-9, and Caspase-3) expression in them. In addition, naringin pretreatment inhibits UVB-stimulated matrix metalloproteinase (MMP-2, MMP-9 and MMP-13) expression in these 3T3 cells. Therefore, we report that naringin can effectively avert UVB-mediated DNA damage, photoaging, and apoptosis in NIH-3T3 cells.
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D'Orazio J, Jarrett S, Amaro-Ortiz A, Scott T. UV radiation and the skin. Int J Mol Sci. 2013;14:12222-48.
-
Balupillai A, Nagarajan RP, Ramasamy K, Govindasamy K, Muthusamy G. Caffeic acid prevents UVB radiation induced photocarcinogenesis through regulation of PTEN signaling in human dermal fibroblasts and mouse skin. Toxicol Appl Pharmacol. 2018;352:87-96.
-
Ramasamy K, Shanmugam M, Balupillai A, Govindhasamy K, Gunaseelan S, Muthusamy G, Robert BM, Nagarajan RP. Ultraviolet radiation-induced carcinogenesis: Mechanisms and experimental models. J Rad Can Res. 2017;8:4.
-
Balupillai A, Prasad RN, Ramasamy K, Muthusamy G, Shanmugham M, Govindasamy K. Caffeic acid inhibits UVB-induced inflammation and photocarcinogenesis through activation of peroxisome proliferator-activated receptor-y in mouse skin. Photochem Photobiol. 2015;91:1458-68.
-
Khalil C, Shebaby D. UVB damage onset and progression 24 h post exposure in human-derived skin cells. Toxicol Rep. 2017;1:441-449.
-
Britto SM, Shanthakumari D, Agilan B, Radhiga T, Kanimozhi G, Prasad NR. Apigenin prevents ultraviolet-B radiation induced cyclobutane pyrimidine dimers formation in human dermal fibroblasts. Mutat Res. 2017; 821:28-35.
-
Denning MF, Wang Y, Tibudan S, Alkan S. Caspase activation and disruption of mitochondrial membrane potential during UV radiation-induced apoptosis of human keratinocytes requires activation of protein kinase C. Cell Death Differ. 2002;9:40.
-
Muzaffer U, Paul VI, Balupillai A, Prasad NR. Protective effect of Juglans regia L., against ultraviolet-B induced photoaging in human epidermal keratinocytes. Biomed Pharmacother. 2019;111:724-732.
-
Gunaseelan S, Balupillai A, Govindasamy K. Linalool prevents oxidative stress activated protein kinase in single UVB exposed UVB cells. PLoS One. 2017;12(5).
-
Surh YJ. Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer. 2003;3(10):768.
-
Singh RP, Agarwal R. Mechanisms and preclinical efficacy of silibinin in preventing skin cancer. Eur J Cancer. 2005;41:1969-79.
-
Nijveldt RJ, Van Nood EL, Van Hoorn DE, Boelens PG, Van Norren K, Van Leeuwen PA. Flavonoids: A review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001;74: 418-25.
-
Kaul TN, Middleton EJ, Ogra PL. Antiviral effect of flavonoids on human viruses. J Med Virol. 1985;15:71-9.
-
Itoh K, Masuda M, Naruto S, Murata K, Matsuda H. Anti-allergic activity of unripe Citrus hassaku fruits extract and its flavanone glycosides on chemical substance-induced dermatitis in mice. J Nat Med. 2009;63:443-50.
-
Kanno S, Shouji A, Asou K, Ishikawa M. Effects of naringin on hydrogen peroxide-induced cytotoxicity and apoptosis in P388 cells. J Pharmacol Sci. 2003;92: 166-70.
-
NilamberLal Das R, Muruhan S, Nagarajan RP, Balupillai A. Naringin prevents ultraviolet-B radiation-induced oxidative damage and inflammation through activation of peroxisome proliferator-activated receptor y in mouse embryonic fibroblast (NIH-3T3) cells. J Biochem Mol Toxicol. 2019;33(3):e22263.
-
Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988;175(1): 184-91.
-
Agilan B, Rajendra Prasad N, Kanimozhi G, Karthikeyan R, Ganesan M, Mohana S, Velmurugan D, Ananthakrishnan D. Caffeic acid inhibits chronic UVB-induced cellular proliferation through JAK-STAT 3 signaling in mouse skin. Photochem Photobiol. 2016;92(3):467-74.
-
Bachman J. Reverse-transcription PCR (rt-PCR). In: Methods in enzymology. Vol. 530. Academic Press; 2013. p. 67-74.
-
Tripoli E, La Guardia M, Giammanco S, Di Majo D, Giammanco M. Citrus flavonoids: Molecular structure, biological activity and nutritional properties: A review. Food Chem. 2007;104(2):466-79.
-
CariNo-Cortes R, Alvarez-Gonzalez I, Martino-Roaro L, Madrigal-Bujaidar E. Effect of naringin on the DNA damage induced by daunorubicin in mouse hepatocytes and cardiocytes. Biol Pharm Bull. 2010;33(4):697-701.
-
Jagetia A, Jagetia GC, Jha S. Naringin, a grapefruit flavanone, protects V79 cells against the bleomycin-induced genotoxicity and decline in survival. J Appl Toxicol. 2007;27(2):122-32.
-
Das S, Das J, Paul A, Samadder A, Khuda-Bukhsh AR. Apigenin, a bioactive flavonoid from Lycopodium clavatum, stimulates nucleotide excision repair genes to protect skin keratinocytes from ultraviolet B-induced reactive oxygen species and DNA damage. J Acupunct Meridian Stud. 2013;6(5):252-62.
-
Guillermo-Lagae R, Deep G, Ting H, Agarwal C, Agarwal R. Silibinin enhances the repair of ultraviolet B-induced DNA damage by activating p53-dependent nucleotide excision repair mechanism in human dermal fibroblasts. Oncotarget. 2015;6(37):39594.
-
Ho JN, Lee YH, Lee YD, Jun WJ, Kim HK, Hong BS, Shin DH, Cho HY. Inhibitory effect of Aucubin isolated from Eucommia ulmoides against UVB-induced matrix metalloproteinase-1 production in human skin fibroblasts. Biosci Biotech Bioch. 2005;69:2227-31.
-
Scharffetter-Kochanek K, Brenneisen P, Wenk J, Herrmann G, Ma W, Kuhr L, Meewes C, Wlaschek M. Photoaging of the skin from phenotype to mechanisms. Exp Gerontol. 2000;35:307-16.
-
Pittayapruek P, Meephansan J, Prapapan O, Komine M, Ohtsuki M. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int J Mol Sci. 2016;17(6):868.
-
Lee EJ, Kim DI, Kim WJ, Moon SK. Naringin inhibits matrix metalloproteinase-9 expression and AKT phosphorylation in tumor necrosis factor-a-induced vascular smooth muscle cells. Mol Nut Food Res. 2009;53(12):1582-91.
-
Aroui S, Aouey B, Chtourou Y, Meunier AC, Fetoui H, Kenani A. Naringin suppresses cell metastasis and the expression of matrix metalloproteinases (MMP-2 and MMP-9) via the inhibition of ERK-P38-JNK signaling pathway in human glioblastoma. Chemico-Biol Int. 2016; 244:195-203.
-
Lee CH, Wu SB, Hong CH, Yu HS, Wei YH. Molecular mechanisms of UV-induced apoptosis and its effects on skin residential cells: The implication in UV-based phototherapy. Int J Mol Sci. 2013;14(3):6414-35.
-
Assefa Z, Van Laethem A, Garmyn M, Agostinis P. Ultraviolet radiation-induced apoptosis in keratinocytes: On the role of cytosolic factors. Biochim Biophys Acta Rev Cancer. 2005;1755(2):90-106.
-
Tzifi F, Economopoulou C, Gourgiotis D, Ardavanis A, Papageorgiou S, Scorilas A. The role of BCL2 family of apoptosis regulator proteins in acute and chronic leukemias. Adv Hematol. 2012;2012.
-
Kim HJ, Song JY, Park HJ, Park HK, Yun DH, Chung JH. Naringin protects against rotenone-induced apoptosis in human neuroblastoma SH-SY5Y cells. Korean J Physiol Pharmacol. 2009;13(4):281-5.
-
Chtourou Y, Aouey B, Kebieche M, Fetoui H. Protective role of naringin against cisplatin induced oxidative stress, inflammatory response and apoptosis in rat striatum via suppressing ROS-mediated NF-KB and P53 signaling pathways. Chemico-Biol Int. 2015;239:76-86.
-
Dong D, Xu L, Yin L, Qi Y, Peng J. Naringin prevents carbon tetrachloride-induced acute liver injury in mice. J Fun Foods. 2015;12:179-91.
-
Raghu Shamprasad Varija, Kudva Avinash Kundadka, Rao Suresh, Prasad Krishna, Mudgal Jayesh, Baliga Manjeshwar Shrinath, Dietary agents in mitigating chemotherapy-related cognitive impairment (chemobrain or chemofog): first review addressing the benefits, gaps, challenges and ways forward, Food & Function, 12, 22, 2021. Crossref