RT Journal Article ID 7ff8af633a7b81c9 A1 Vargas-del-Rlo, L.M. A1 Montoya, Sandra A1 Sepulveda-Arias, J.C. T1 Preserving and Maintaining Culinary-Medicinal Royal Sun Mushroom, Agaricus brasiliensis (Agaricomycetes), in Sterile Distilled Water JF International Journal of Medicinal Mushrooms JO IJM YR 2017 FD 2017-07-03 VO 19 IS 5 SP 467 OP 475 K1 Agaricus brasiliensis K1 growth rate K1 medicinal mushrooms K1 sterile distilled water K1 strain conservation K1 strain maintenance AB Strains of Agaricus brasiliensis require special procedures for conservation. Thus, the objective of this research was to establish conservation and maintenance procedures A. brasiliensis strain PSWC838 from the University of Pennsylvania (ABWC838) and an A. brasiliensis strain from the Fujian Agriculture and Forestry University (ABC). The medium in which mycelia grew the quickest for both strains was selected using a multifactorial design with 2 strains, 4 culture media, and incubation for 5 different times; the growth rate (mm/day) was the response variable. Analysis of variance showed that the potato dextrose agar (PDA) medium and potato extract did not display a significantly different growth rate, and PDA was selected for safety reasons. We also evaluated the viability of the strains grown on PDA and 0.2% activated carbon after 3 months of storage in sterile distilled water. A factorial design was applied with 2 factors, the strain and incubation for 10 different times. The Tukey post hoc test indicated that ABC showed quicker and more homogeneous growth than ABWC838. Finally, the results showed that pieces of mycelium of ABC and ABWC838 strains inoculated on the PDA medium with 0.2% activated carbon and preserved in sterile distilled water at 18 ± 1°C showed 100% viability after 3 months of storage. Moreover, the results of semiquantitative biochemical tests confirmed that the production of laccases and amylases was preserved in these strains after storage in sterile water, enhancing their ability to degrade substrates containing lignin and starchy waste. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,77c3ff4726898ae1,7ff8af633a7b81c9.html