RT Journal Article
ID 686df57a68f8dd60
A1 Ye, Libin
A1 Zheng, Xiaolin
A1 Zhang, Jingsong
A1 Yang, Yan
A1 Meng, Yuecheng
A1 Li, Jianrong
A1 Chen, Wei
A1 Li, Ang
A1 Pan, Ying-jie
T1 Composition Analysis and Immunomodulatory Capacity of Peptidoglycan from Ling Zhi or Reishi Medicinal Mushroom, <i>Ganoderma lucidum</i> (W. Curt.: Fr.) P. Karst. Strain 119 (Aphyllophoromycetideae)
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2010
FD 2010-05-26
VO 12
IS 2
SP 157
OP 165
K1 medicinal mushrooms
K1 Ling Zhi
K1 Reishi mushroom
K1 Ganoderma lucidum
K1 composition analysis
K1 water-soluble peptidoglycan
K1 immunomodulatory activity
K1 gas chromatography-mass spectrum
AB LZ-B-1, a water-soluble peptidoglycan, was prepared from the fruiting bodies of Ling Zhi or Reishi medicinal mushroom, <i>Ganoderma lucidum</i>, by ultrafiltration, DEAE-Sepharose Fast-Flow column, and Sepharose CL-6B column. To develop functional food or pharmaceuticals using LZ-B-1 and to investigate the relationship between the structure and bioactivity of LZ-B-1, the composition and immunomodulatory capacity of LZ-B-1 were studied herein. The peptidoglycan had a relative molecular weight of 1.12 &times; 10<sup>4</sup> Da, and total carbohydrate and peptide percentages were determined to be &sim;94.2% and &sim;5.4%, respectively. The carbohydrate moiety was mainly composed of L-fucose, D-galatose, and D-glucose in a molar ratio of approximately 1:5:2, together with small amounts of L-arabinose and D-mannose. The peptide moiety mainly contained arginine, threonine, glycine, serine, and aspartic acid in a molar ratio of approximately 1:1:2:2:1. Methylation reaction and GC-MS analysis indicated that the carbohydrate moiety mainly consisted of 1,6-disubstituted-galactopyranosyl, 1,2,6-trisubstituted-galactopyranosyl, 1-substituted-fucopyranosyl, 1,3-disubstituted- glucopyranosyl, 1,4,6-trisubstituted-glucopyranosyl, and 1-substituted-glucopyranosyl. Mouse spleen cell proliferation assay <i>in vitro</i> showed that a concentration of 200 &mu;g/mL of LZ-B-1 was the optimum concentration to promote the proliferation of mouse spleen lymphocytes (MSLs). In general, the higher the mouse spleen cell proliferation rate, the stronger the immunomodulatory activity. How the peptidoglycan stimulates the proliferation of MSLs and the bioactivity-structure relationship requires more research.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,40aa21637478b2ab,686df57a68f8dd60.html