%0 Journal Article %A Grzywacz, Agata %A Argasinska, Joanna Gdula %A Kala, Katarzyna %A Opoka, Wlodzimierz %A Muszynska, Bozena %D 2016 %I Begell House %K anti-inflammatory properties, Imleria badia, medicinal mushrooms, mushroom in vitro cultures, zinc, zinc hydroaspartate %N 9 %P 769-779 %R 10.1615/IntJMedMushrooms.v18.i9.20 %T Anti-Inflammatory Activity of Biomass Extracts of the Bay Mushroom, Imleria badia (Agaricomycetes), in RAW 264.7 Cells %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2e89624a4c27b9d4,502f76be48dc5c0b.html %V 18 %X The popular edible bay mushroom, Imleria badia, is rich in phenolic and indole compounds, unsaturated fatty acids, and elements that have anti-inflammatory activity, including zinc. The aim of this study was to evaluate the pro- or anti-inflammatory impact of I. badia biomass extracts on RAW 264.7 cells. We analyzed the influence of I. badia extracts and/or lipopolysaccharide (LPS) cotreatment on the fatty acid profile of cell membranes. We also analyzed cyclooxygenase-2, prostaglandin E synthase, glutathione S transferase Mul, and nuclear factor (NF)-κB p50 and p65 protein expression, as well as the activity of peroxisome proliferator-activated receptor γ and NF-κB transcription factors. Incubation of cells with I. badia extracts resulted in a decreased expression of cyclooxygenase-2 and prostaglandin E2 synthase when compared with the LPS-activated macrophages. Glutathione S transferase Mul expression increased after incubation of cells with I. badia extracts. p50 And p65 NF-κB protein levels were lower in macrophages after extract treatment. Lower activity of NF-κB and higher activity of peroxisome proliferator-activated receptor γ was observed in cells treated with I. badia extracts in the presence of LPS when compared with cells activated by LPS alone. Our findings suggest the anti-inflammatory properties of I. badia biomass extracts from in vitro cultures, which could contribute to the development of new strategies in the treatment of inflammation. %8 2016-11-18