%0 Journal Article %A Du, Ping %A Cui, Bao-Kai %A Dai, Yu-Cheng %D 2011 %I Begell House %K medicinal mushrooms, Auricularia polytricha, gene flow, genetic differentiation, Nei’s gene diversity, population, cultivated strains %N 3 %P 289-298 %R 10.1615/IntJMedMushr.v13.i3.90 %T High Genetic Diversity in Wild Culinary-Medicinal Wood Ear Mushroom, Auricularia polytricha (Mont.) Sacc., in Tropical China Revealed by ISSR Analysis %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,44cae58e6753b88e,4ed6b4d533ade59f.html %V 13 %X The genetic diversity of 40 wild Auricularia polytricha strains within ten natural populations in tropical China and five cultivated strains were analyzed by using twelve inter-simple sequence repeat (ISSR) primers. At the species level, the percentage of polymorphic loci (P) = 99.8%, the effective number of alleles (Ne) = 1.3569, Nei’s gene diversity (H) = 0.2398, Shannon information index (I) = 0.3896, and total genetic diversity (Ht) = 0.2346 indicate a high level of genetic diversity in wild A. polytricha. At the population level of the wild fungus, P = 43.51%, Ne = 1.2675, H = 0.1702, I = 0.2504. However, the genetic diversity of cultivated strains was the lowest of all populations (Ne = 1.1584, H = 0.0940, I = 0.1440). A moderate degree of genetic differentiation (Gst = 0.347) among the sampled wild populations was detected based on Nei’s gene diversity analysis, suggesting that 65.3% of variation existed within this population. The high genetic variation level within wild populations may mostly result from a low level of gene flow (Nm = 0.9408) and random genetic drift. %8 2011-06-22